› 3rvm

X-ray diffraction
1.45Å resolution

Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D and E89R

Released:
DOI: 10.2210/pdb3rvm/pdb
PDB entry 3rvm coloured by chain, front view.
PDB entry 3rvm coloured by chain, side view.
PDB entry 3rvm coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.
Immormino RM, Starbird CA, Silversmith RE, Bourret RB
Biochemistry 54 3514-27 (2015)
PMID: 25928369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 14.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE67 (Residues: 1-129; Coverage: 100%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 45.328Å b: 47.019Å c: 53.349Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.139 0.166
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Microbial 5'-nucleotidases: their characteristics, roles in cellular metabolism, and possible practical applications.
Zakataeva NP. (2021)
1 more

1 mention without citation

Probing Mechanistic Similarities between Response Regulator Signaling Proteins and Haloacid Dehalogenase Phosphatases.
Immormino et al. (2015)