› 3rsk

X-ray diffraction
2Å resolution

STRUCTURE OF THE K7A/R10A/K66A VARIANT OF RIBONUCLEASE A

Released:
DOI: 10.2210/pdb3rsk/pdb
PDB entry 3rsk coloured by chain, front view.
PDB entry 3rsk coloured by chain, side view.
PDB entry 3rsk coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Coulombic effects of remote subsites on the active site of ribonuclease A.
Fisher BM, Schultz LW, Raines RT
Biochemistry 37 17386-401 (1998)
PMID: 9860854

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.51 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3221
Unit cell:
a: 68.15Å b: 68.15Å c: 65.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 not available not available
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Intramolecular interactions at protein surfaces and their impact on protein function.
Robertson AD. (2002)
41 other articles

3 mentions without citation

Hypervalent nonbonded interactions of a divalent sulfur atom. Implications in protein architecture and the functions.
Iwaoka et al. (2012)
2 more