3rj0

X-ray diffraction
2.54Å resolution

Plant steroid receptor BRI1 ectodomain in complex with brassinolide

Released:

Function and Biology Details

Reactions catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-127636 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Protein BRASSINOSTEROID INSENSITIVE 1 Chain: A
Molecule details ›
Chain: A
Length: 772 amino acids
Theoretical weight: 83.49 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O22476 (Residues: 29-788; Coverage: 65%)
Gene names: At4g39400, BRI1, F23K16.30
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 175.17Å b: 67.236Å c: 119.246Å
α: 90° β: 121.42° γ: 90°
R-values:
R R work R free
0.187 0.184 0.241
Expression system: Trichoplusia ni