› 3rap

X-ray diffraction
2.2Å resolution

The small G protein Rap2 in a non catalytic complex with GTP

Released:
DOI: 10.2210/pdb3rap/pdb
PDB entry 3rap coloured by chain, front view.
PDB entry 3rap coloured by chain, side view.
PDB entry 3rap coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the small G protein Rap2 in a non-catalytic complex with GTP.
Ménétrey J, Cherfils J
Proteins 37 465-73 (1999)
PMID: 10591105

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145173 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rap-2a Chains: R, S
Molecule details ›
Chains: R, S
Length: 167 amino acids
Theoretical weight: 18.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10114 (Residues: 1-167; Coverage: 91%)
Gene name: RAP2A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P21
Unit cell:
a: 41.01Å b: 59.83Å c: 68.44Å
α: 90° β: 97.07° γ: 90°
R-values:
R R work R free
0.211 0.211 0.262
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
7 other articles

8 mentions without citation

The structural GDP/GTP cycle of human Arf6.
Pasqualato et al. (2001)
7 more