› 3rab

X-ray diffraction
2Å resolution

GPPNHP-BOUND RAB3A AT 2.0 A RESOLUTION

Released:
DOI: 10.2210/pdb3rab/pdb
PDB entry 3rab coloured by chain, front view.
PDB entry 3rab coloured by chain, side view.
PDB entry 3rab coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of activation and GTP hydrolysis in Rab proteins.
Dumas JJ, Zhu Z, Connolly JL, Lambright DG
Structure 7 413-23 (1999)
PMID: 10196122

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-3A Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.66 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63012 (Residues: 18-186; Coverage: 77%)
Gene name: Rab3a
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 88.9Å b: 35Å c: 58.6Å
α: 90° β: 107.2° γ: 90°
R-values:
R R work R free
0.191 0.191 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The Rab GTPase family.
Stenmark et al. (2001)
13 more

15 mentions without citation

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
14 more