› 3r7g

X-ray diffraction
2.2Å resolution

Crystal structure of Spire KIND domain in complex with the tail of FMN2

Released:
DOI: 10.2210/pdb3r7g/pdb
PDB entry 3r7g coloured by chain, front view.
PDB entry 3r7g coloured by chain, side view.
PDB entry 3r7g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Spire KIND domain and insights into its interaction with Fmn-family formins
Vizcarra C, Kreutz B, Rodal A, Toms A, Lu J, Zheng W, Quinlan M, Eck M
Proc. Natl. Acad. Sci. U.S.A. (2011)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170714 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein spire homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08AE8 (Residues: 20-237; Coverage: 29%)
Gene names: KIAA1135, SPIR1, SPIRE1
Sequence domains: Kinase non-catalytic C-lobe domain
Structure domains: Transferase(Phosphotransferase) domain 1
Formin-2 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NZ56 (Residues: 1701-1722; Coverage: 1%)
Gene name: FMN2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P43212
Unit cell:
a: 66.444Å b: 66.444Å c: 116.397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.229
Expression system: Escherichia coli

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