› 3qz1

X-ray diffraction
3Å resolution

Crystal Structure of Bovine Steroid of 21-hydroxylase (P450c21)

Released:
DOI: 10.2210/pdb3qz1/pdb
PDB entry 3qz1 coloured by chain, front view.
PDB entry 3qz1 coloured by chain, side view.
PDB entry 3qz1 coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Zhao B, Waterman MR

Function and Biology Details

Reaction catalysed: 
17-alpha-hydroxyprogesterone + [reduced NADPH--hemoprotein reductase] + O(2) = 11-deoxycortisol + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid 21-hydroxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 496 amino acids
Theoretical weight: 56.16 KDa
Source organism: Bos taurus
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P00191 (Residues: 1-496; Coverage: 100%)
Gene names: CYP21, CYP21A1, CYP21A2
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:
Ligand 3QZ 8 x 3QZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G, APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 67.871Å b: 167.998Å c: 111.843Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.282 0.282 0.297
Expression system: Escherichia coli DH5[alpha]

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