› 3qxt

X-ray diffraction
1.7Å resolution

Structure of an Anti-Methotrexate CDR1-3 Graft VHH Antibody in Complex with Methotrexate

Released:
DOI: 10.2210/pdb3qxt/pdb
PDB entry 3qxt coloured by chain, front view.
PDB entry 3qxt coloured by chain, side view.
PDB entry 3qxt coloured by chain, top view.
Source organism: Lama glama
Primary publication:
An anti-hapten camelid antibody reveals a cryptic binding site with significant energetic contributions from a nonhypervariable loop.
Fanning SW, Horn JR
Protein Sci 20 1196-207 (2011)
PMID: 21557375

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anti-Methotrexate CDR1-3 Graft VHH Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.7 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand MTX 2 x MTX
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6122
Unit cell:
a: 103.063Å b: 103.063Å c: 157.073Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

VHH antibodies: emerging reagents for the analysis of environmental chemicals.
Bever et al. (2016)
6 more

3 mentions without citation

Structure and development of single domain antibodies as modules for therapeutics and diagnostics.
Hoey et al. (2019)
2 more