› 3qx8

X-ray diffraction
2.3Å resolution

Crystal structure of MID domain from hAGO2 in complex with m7GpppG

Released:
DOI: 10.2210/pdb3qx8/pdb
PDB entry 3qx8 coloured by chain, front view.
PDB entry 3qx8 coloured by chain, side view.
PDB entry 3qx8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of 5'-mRNA-cap interactions with the human AGO2 MID domain.
Frank F, Fabian MR, Stepinski J, Jemielity J, Darzynkiewicz E, Sonenberg N, Nagar B
EMBO Rep 12 415-20 (2011)
PMID: 21475248

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 15.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKV8 (Residues: 439-575; Coverage: 16%)
Gene names: AGO2, EIF2C2
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GTG 3 x GTG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 40.686Å b: 46.967Å c: 66.119Å
α: 86.09° β: 73.33° γ: 83.38°
R-values:
R R work R free
0.226 0.226 0.279
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The mechanics of miRNA-mediated gene silencing: a look under the hood of miRISC.
Fabian et al. (2012)
4 more

1 mention without citation

Structural analysis of 5'-mRNA-cap interactions with the human AGO2 MID domain.
Frank et al. (2011)