› 3qty

X-ray diffraction
1.8Å resolution

Crystal structure of Phosphoribosylaminoimidazole Synthetase from Francisella tularensis complexed with pyrophosphate

Released:
DOI: 10.2210/pdb3qty/pdb
PDB entry 3qty coloured by chain, front view.
PDB entry 3qty coloured by chain, side view.
PDB entry 3qty coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis
Entry authors: Maltseva N, Kim Y, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylformylglycinamidine cyclo-ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 38.75 KDa
Source organism: Francisella tularensis subsp. tularensis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5NGF2 (Residues: 1-347; Coverage: 100%)
Gene names: FTT_0893, purM
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 1 x SO4
Ligand TRS 3 x TRS
Ligand POP 3 x POP
Ligand PO4 2 x PO4
Ligand FMT 2 x FMT
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 65.482Å b: 88.721Å c: 112.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.202
Expression system: Escherichia coli BL21

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