› 3qr8

X-ray diffraction
2.03Å resolution

Crystal structure of the bacteriophage P2 membrane-piercing protein gpV

Released:
DOI: 10.2210/pdb3qr8/pdb
PDB entry 3qr8 coloured by chain, front view.
PDB entry 3qr8 coloured by chain, side view.
PDB entry 3qr8 coloured by chain, top view.
Source organism: Escherichia virus P2
Primary publication:
Phage pierces the host cell membrane with the iron-loaded spike.
Browning C, Shneider MM, Bowman VD, Schwarzer D, Leiman PG
Structure 20 326-39 (2012)
PMID: 22325780

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spike protein Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 22.5 KDa
Source organism: Escherichia virus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: P31340 (Residues: 1-211; Coverage: 100%)
Gene name: V
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P321
Unit cell:
a: 68.539Å b: 68.539Å c: 132.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.26 0.305
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Deadly syringes: type VI secretion system activities in pathogenicity and interbacterial competition.
Kapitein et al. (2013)
11 more

4 mentions without citation

Keeping It Together: Structures, Functions, and Applications of Viral Decoration Proteins.
Dedeo et al. (2020)
3 more