› 3qn3

X-ray diffraction
2.13Å resolution

Phosphopyruvate hydratase from Campylobacter jejuni.

Released:
DOI: 10.2210/pdb3qn3/pdb
PDB entry 3qn3 coloured by chain, front view.
PDB entry 3qn3 coloured by chain, side view.
PDB entry 3qn3 coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Osipiuk J, Gu M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo octamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 417 amino acids
Theoretical weight: 45.26 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42448 (Residues: 1-414; Coverage: 100%)
Gene names: Cj1672c, eno
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand MPD 4 x MPD
Ligand GOL 3 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I222
Unit cell:
a: 120.47Å b: 148.886Å c: 234.719Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.216
Expression system: Escherichia coli BL21(DE3)

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