› 3qks

X-ray diffraction
2.1Å resolution

Mre11 Rad50 binding domain bound to Rad50

Released:
DOI: 10.2210/pdb3qks/pdb
PDB entry 3qks coloured by chain, front view.
PDB entry 3qks coloured by chain, side view.
PDB entry 3qks coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair.
Williams GJ, Williams RS, Williams JS, Moncalian G, Arvai AS, Limbo O, Guenther G, SilDas S, Hammel M, Russell P, Tainer JA
Nat Struct Mol Biol 18 423-31 (2011)
PMID: 21441914

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157666 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA double-strand break repair Rad50 ATPase Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 23.62 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P58301 (Residues: 1-195; Coverage: 22%)
Gene names: PF1167, rad50
Sequence domains: AAA domain
Structure domains:
DNA double-strand break repair Rad50 ATPase Chain: B
Molecule details ›
Chain: B
Length: 179 amino acids
Theoretical weight: 20.55 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P58301 (Residues: 704-882; Coverage: 20%)
Gene names: PF1167, rad50
Sequence domains: AAA domain, putative AbiEii toxin, Type IV TA system
Structure domains: P-loop containing nucleotide triphosphate hydrolases
DNA double-strand break repair protein Mre11 Chain: C
Molecule details ›
Chain: C
Length: 34 amino acids
Theoretical weight: 4.06 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1N9 (Residues: 348-381; Coverage: 8%)
Gene names: PF1166, mre11

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 55.779Å b: 91.232Å c: 107.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.256
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Double-strand break end resection and repair pathway choice.
Symington et al. (2011)
43 more

10 mentions without citation

Envisioning the dynamics and flexibility of Mre11-Rad50-Nbs1 complex to decipher its roles in DNA replication and repair.
Lafrance-Vanasse et al. (2015)
9 more