› 3qi6

X-ray diffraction
1.91Å resolution

Crystal Structure of Cystathionine gamma-synthase MetB (Cgs) from Mycobacterium ulcerans Agy99

Released:
DOI: 10.2210/pdb3qi6/pdb
PDB entry 3qi6 coloured by chain, front view.
PDB entry 3qi6 coloured by chain, side view.
PDB entry 3qi6 coloured by chain, top view.
Source organism: Mycobacterium ulcerans Agy99
Primary publication:
Structure of the cystathionine γ-synthase MetB from Mycobacterium ulcerans.
Clifton MC, Abendroth J, Edwards TE, Leibly DJ, Gillespie AK, Ferrell M, Dieterich SH, Exley I, Staker BL, Myler PJ, Van Voorhis WC, Stewart LJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1154-8 (2011)
PMID: 21904066

Function and Biology Details

Reaction catalysed: 
O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine gamma-synthase MetB (Cgs) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 41.29 KDa
Source organism: Mycobacterium ulcerans Agy99
Expression system: Escherichia coli
UniProt:
  • Canonical: A0PKT3 (Residues: 1-388; Coverage: 100%)
Gene names: MUL_0201, metB
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand NA 1 x NA
Ligand GOL 2 x GOL
Ligand EDO 1 x EDO
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 80.956Å b: 106.924Å c: 100.307Å
α: 90° β: 113.67° γ: 90°
R-values:
R R work R free
0.202 0.2 0.241
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Catalytic specificity of the Lactobacillus plantarum cystathionine γ-lyase presumed by the crystallographic analysis.
Matoba et al. (2020)
2 more

4 mentions without citation

Comparative genomics analysis of Mycobacterium ulcerans for the identification of putative essential genes and therapeutic candidates.
Butt et al. (2012)
3 more