› 3qe0

X-ray diffraction
3Å resolution

A Galpha-i1 P-loop mutation prevents transition to the activated state

Released:
DOI: 10.2210/pdb3qe0/pdb
PDB entry 3qe0 coloured by chain, front view.
PDB entry 3qe0 coloured by chain, side view.
PDB entry 3qe0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A P-loop mutation in Gα subunits prevents transition to the active state: implications for G-protein signaling in fungal pathogenesis.
Bosch DE, Willard FS, Ramanujam R, Kimple AJ, Willard MD, Naqvi NI, Siderovski DP
PLoS Pathog 8 e1002553 (2012)
PMID: 22383884

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-158957 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 325 amino acids
Theoretical weight: 37.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63096 (Residues: 33-354; Coverage: 91%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
KB752 peptide Chains: F, G
Molecule details ›
Chains: F, G
Length: 16 amino acids
Theoretical weight: 2.04 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand GDP 3 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 106.637Å b: 106.637Å c: 455.062Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.247 0.292
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

Structural features of the G-protein/GPCR interactions.
Moreira IS. (2014)
1 more

1 mention without citation

A P-loop mutation in Gα subunits prevents transition to the active state: implications for G-protein signaling in fungal pathogenesis.
Bosch et al. (2012)