› 3q81

X-ray diffraction
2Å resolution

Imipenem acylated BlaR1 sensor domain from Staphylococcus aureus

Released:
DOI: 10.2210/pdb3q81/pdb
PDB entry 3q81 coloured by chain, front view.
PDB entry 3q81 coloured by chain, side view.
PDB entry 3q81 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Lysine Nzeta-decarboxylation switch and activation of the beta-lactam sensor domain of BlaR1 protein of methicillin-resistant Staphylococcus aureus.
Borbulevych O, Kumarasiri M, Wilson B, Llarrull LI, Lee M, Hesek D, Shi Q, Peng J, Baker BM, Mobashery S
J Biol Chem 286 31466-72 (2011)
PMID: 21775440

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein BlaR1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 29.59 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18357 (Residues: 332-368, 371-371, 373-583; Coverage: 43%)
Gene name: blaR1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand IM2 2 x IM2
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 46.651Å b: 107.581Å c: 56.276Å
α: 90° β: 108.29° γ: 90°
R-values:
R R work R free
0.191 0.188 0.237
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial cell-wall recycling.
Johnson et al. (2013)
4 more

5 mentions without citation

New insights into the mechanism of odorant detection by the malaria-transmitting mosquito Anopheles gambiae.
Davrazou et al. (2011)
4 more