3pca Summary

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STRUCTURE OF PROTOCATECHUATE 3,4-DIOXYGENASE COMPLEXED WITH 3,4-DIHYDROXYBENZOATE

The structure was published by Orville, A.M., Lipscomb, J.D., and Ohlendorf, D.H., in 1997 in a paper entitled "Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase: endogenous Fe3+ ligand displacement in response to substrate binding." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 1997.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely PROTOCATECHUATE 3,4-DIOXYGENASE.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The most likely quaternary structure is a 24meric assembly.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A PROTOCATECHUATE 3,4-DIOXYGENASE P00436 (2-201) (PCXA_PSEPU)search Pseudomonas putidasearch 100% 200 100%
B PROTOCATECHUATE 3,4-DIOXYGENASE P00436 (2-201) (PCXA_PSEPU)search Pseudomonas putidasearch 100% 200 100%
C PROTOCATECHUATE 3,4-DIOXYGENASE P00436 (2-201) (PCXA_PSEPU)search Pseudomonas putidasearch 100% 200 100%
D PROTOCATECHUATE 3,4-DIOXYGENASE P00436 (2-201) (PCXA_PSEPU)search Pseudomonas putidasearch 100% 200 100%
E PROTOCATECHUATE 3,4-DIOXYGENASE P00436 (2-201) (PCXA_PSEPU)search Pseudomonas putidasearch 100% 200 100%
F PROTOCATECHUATE 3,4-DIOXYGENASE P00436 (2-201) (PCXA_PSEPU)search Pseudomonas putidasearch 100% 200 100%
M PROTOCATECHUATE 3,4-DIOXYGENASE P00437 (2-239) (PCXB_PSEPU)search Pseudomonas putidasearch 100% 238 97%
N PROTOCATECHUATE 3,4-DIOXYGENASE P00437 (2-239) (PCXB_PSEPU)search Pseudomonas putidasearch 100% 238 97%
O PROTOCATECHUATE 3,4-DIOXYGENASE P00437 (2-239) (PCXB_PSEPU)search Pseudomonas putidasearch 100% 238 97%
P PROTOCATECHUATE 3,4-DIOXYGENASE P00437 (2-239) (PCXB_PSEPU)search Pseudomonas putidasearch 100% 238 97%
Q PROTOCATECHUATE 3,4-DIOXYGENASE P00437 (2-239) (PCXB_PSEPU)search Pseudomonas putidasearch 100% 238 97%
R PROTOCATECHUATE 3,4-DIOXYGENASE P00437 (2-239) (PCXB_PSEPU)search Pseudomonas putidasearch 100% 238 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00436 (2 - 201) PROTOCATECHUATE 3,4-DIOXYGENASE Pseudomonas putida
P00437 (2 - 239) PROTOCATECHUATE 3,4-DIOXYGENASE Pseudomonas putida

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D, E, F (P00436) Aromatic compound dioxygenasesearch Protocatechuate 3,4-Dioxygenase, subunit Asearch PF00775: Dioxygenasesearch
M, N, O, P, Q, R (P00437) Aromatic compound dioxygenasesearch Protocatechuate 3,4-Dioxygenase, subunit Asearch PF00775: Dioxygenasesearch, PF12391: Protocatechuate 3,4-dioxygenase beta subunit N terminalsearch

Chain ID Biological process (GO) Molecular function (GO)
A, B, C, D, E, F (P00436) cellular aromatic compound metabolic processsearch oxidation-reduction processsearch aromatic compound catabolic processsearch beta-ketoadipate pathwaysearch iron ion bindingsearch oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygensearch catalytic activitysearch ferric iron bindingsearch protocatechuate 3,4-dioxygenase activitysearch oxidoreductase activitysearch dioxygenase activitysearch
M, N, O, P, Q, R (P00437) protocatechuate catabolic processsearch oxidation-reduction processsearch cellular aromatic compound metabolic processsearch beta-ketoadipate pathwaysearch aromatic compound catabolic processsearch protocatechuate 3,4-dioxygenase activitysearch catalytic activitysearch iron ion bindingsearch ferric iron bindingsearch dioxygenase activitysearch metal ion bindingsearch oxidoreductase activitysearch oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygensearch

Chain InterPro annotation
A, B, C, D, E, F Intradiol ring-cleavage dioxygenase, C-terminalsearch Protocatechuate 3,4-dioxygenase, alpha subunitsearch Intradiol ring-cleavage dioxygenase, coresearch
M, N, O, P, Q, R Intradiol ring-cleavage dioxygenase, C-terminalsearch Protocatechuate 3,4-dioxygenase, beta subunitsearch Intradiol ring-cleavage dioxygenase, coresearch Protocatechuate 3,4-dioxygenase beta subunit, N-terminalsearch