› 3p8v

X-ray diffraction
1.8Å resolution

Crystal structure of the archaeal asparagine synthetase A complexed with L-Aspartic acid

Released:
DOI: 10.2210/pdb3p8v/pdb
PDB entry 3p8v coloured by chain, front view.
PDB entry 3p8v coloured by chain, side view.
PDB entry 3p8v coloured by chain, top view.
Source organism: Pyrococcus abyssi
Primary publication:
Crystal structure of the archaeal asparagine synthetase: interrelation with aspartyl-tRNA and asparaginyl-tRNA synthetases.
Blaise M, Fréchin M, Oliéric V, Charron C, Sauter C, Lorber B, Roy H, Kern D
J Mol Biol 412 437-52 (2011)
PMID: 21820443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 34.13 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9V228 (Residues: 1-294; Coverage: 100%)
Gene names: PAB2356, asnS-like
Sequence domains: tRNA synthetases class II (D, K and N)
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

1 bound ligand:
Ligand ASP 2 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 58.33Å b: 61.5Å c: 155.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.218
Expression system: Escherichia coli

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Citations

3 review citations

Emergence and evolution.
Bullwinkle et al. (2014)
2 more