3p70 Summary

pdbe.org/3p70
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Structural basis of thrombin-mediated factor V activation: essential role of the hirudin-like sequence Glu666-Glu672 for processing at the heavy chain-B domain junction

The structure was published by Corral-Rodriguez, M.A., Bock, P.E., Hernandez-Carvajal, E., Gutierrez-Gallego, R., and Fuentes-Prior, P., in 2011 in a paper entitled "Structural basis of thrombin-mediated factor V activation: the Glu666-Glu672 sequence is critical for processing at the heavy chain-B domain junction." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.55 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely HUMAN ALPHA-THROMBIN, LIGHT CHAIN, HUMAN ALPHA-THROMBIN, HEAVY CHAIN, and HUMAN FACTOR V, A2-B DOMAIN LINKER.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A HUMAN ALPHA-THROMBIN, LIGHT CHAIN P00734 (328-363) (THRB_HUMAN)search Homo sapienssearch 93% 36 80%
C HUMAN ALPHA-THROMBIN, LIGHT CHAIN P00734 (328-363) (THRB_HUMAN)search Homo sapienssearch 93% 36 80%
E HUMAN ALPHA-THROMBIN, LIGHT CHAIN P00734 (328-363) (THRB_HUMAN)search Homo sapienssearch 93% 36 80%
G HUMAN ALPHA-THROMBIN, LIGHT CHAIN P00734 (328-363) (THRB_HUMAN)search Homo sapienssearch 93% 36 80%
B HUMAN ALPHA-THROMBIN, HEAVY CHAIN P00734 (364-622) (THRB_HUMAN)search Homo sapienssearch 93% 259 98%
D HUMAN ALPHA-THROMBIN, HEAVY CHAIN P00734 (364-622) (THRB_HUMAN)search Homo sapienssearch 93% 259 98%
F HUMAN ALPHA-THROMBIN, HEAVY CHAIN P00734 (364-622) (THRB_HUMAN)search Homo sapienssearch 93% 259 98%
H HUMAN ALPHA-THROMBIN, HEAVY CHAIN P00734 (364-622) (THRB_HUMAN)search Homo sapienssearch 93% 259 98%
M HUMAN FACTOR V, A2-B DOMAIN LINKER P12259 (685-737) (FA5_HUMAN)search Homo sapienssearch < 90% 71 8%
N HUMAN FACTOR V, A2-B DOMAIN LINKER P12259 (685-737) (FA5_HUMAN)search Homo sapienssearch < 90% 71 8%
O HUMAN FACTOR V, A2-B DOMAIN LINKER P12259 (685-737) (FA5_HUMAN)search Homo sapienssearch < 90% 71 8%
P HUMAN FACTOR V, A2-B DOMAIN LINKER P12259 (685-737) (FA5_HUMAN)search Homo sapienssearch < 90% 71 8%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00734 (328 - 363) HUMAN ALPHA-THROMBIN, LIGHT CHAIN Homo sapiens
P12259 (685 - 737) HUMAN FACTOR V, A2-B DOMAIN LINKER Homo sapiens

Chain Sequence family (Pfam)
A, C, E, G (P00734) PF09396: Thrombin light chainsearch
B, D, F, H (P00734) PF00089: Trypsinsearch
M, N, O, P

Chain ID Cellular component (GO) Biological process (GO) Molecular function (GO)
A, C, E, G (P00734) extracellular regionsearch blood coagulationsearch proteolysissearch serine-type endopeptidase activitysearch
B, D, F, H (P00734) proteolysissearch blood coagulationsearch serine-type endopeptidase activitysearch catalytic activitysearch calcium ion bindingsearch

Chain InterPro annotation
A, C, E, G Thrombin light chainsearch
B, D, F, H Peptidase S1search Peptidase S1A, chymotrypsin-typesearch Prothrombin/thrombinsearch Trypsin-like cysteine/serine peptidase domainsearch Peptidase S1, trypsin family, active sitesearch
M, N, O, P