› 3p3c

X-ray diffraction
1.25Å resolution

Crystal Structure of the Aquifex aeolicus LpxC/LPC-009 complex

Released:
DOI: 10.2210/pdb3p3c/pdb
PDB entry 3p3c coloured by chain, front view.
PDB entry 3p3c coloured by chain, side view.
PDB entry 3p3c coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Species-specific and inhibitor-dependent conformations of LpxC: implications for antibiotic design.
Lee CJ, Liang X, Chen X, Zeng D, Joo SH, Chung HS, Barb AW, Swanson SM, Nicholas RA, Li Y, Toone EJ, Raetz CR, Zhou P
Chem Biol 18 38-47 (2011)
PMID: 21167751

Function and Biology Details

Reaction catalysed: 
UDP-3-O-((3R)-3-hydroxyacyl)-N-acetyl-alpha-D-glucosamine + H(2)O = UDP-3-O-((3R)-3-hydroxyacyl)-alpha-D-glucosamine + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-3-O-acyl-N-acetylglucosamine deacetylase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.32 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67648 (Residues: 2-275; Coverage: 97%)
Gene names: aq_1772, envA, lpxC
Sequence domains: UDP-3-O-acyl N-acetylglycosamine deacetylase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand 3P3 1 x 3P3
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P61
Unit cell:
a: 65.604Å b: 65.604Å c: 132.263Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.152 0.161
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Prospects for new antibiotics: a molecule-centered perspective.
Walsh et al. (2014)
12 more

2 mentions without citation

Species-specific and inhibitor-dependent conformations of LpxC: implications for antibiotic design.
Lee et al. (2011)
1 more