› 3p2y

X-ray diffraction
1.82Å resolution

Crystal structure of alanine dehydrogenase/pyridine nucleotide transhydrogenase from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb3p2y/pdb
PDB entry 3p2y coloured by chain, front view.
PDB entry 3p2y coloured by chain, side view.
PDB entry 3p2y coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proton-translocating NAD(P)(+) transhydrogenase Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 39.63 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QNT0 (Residues: 1-360; Coverage: 100%)
Gene name: MSMEG_0152
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand NA 1 x NA
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P6122
Unit cell:
a: 89.76Å b: 89.76Å c: 179.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.196
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more