› 3p19

X-ray diffraction
2.05Å resolution

Improved NADPH-dependent Blue Fluorescent Protein

Released:
DOI: 10.2210/pdb3p19/pdb
PDB entry 3p19 coloured by chain, front view.
PDB entry 3p19 coloured by chain, side view.
PDB entry 3p19 coloured by chain, top view.
Source organism: Vibrio vulnificus
Primary publication:
Structure of a NADPH-dependent blue fluorescent protein revealed the unique role of Gly176 on the fluorescence enhancement.
Kao TH, Chen Y, Pai CH, Chang MC, Wang AH
J Struct Biol 174 485-93 (2011)
PMID: 21397029

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative blue fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 266 amino acids
Theoretical weight: 28.89 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F172 (Residues: 1-239; Coverage: 100%)
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NDP 4 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P43
Unit cell:
a: 64.403Å b: 64.403Å c: 262.588Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.239
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Accessing non-natural reactivity by irradiating nicotinamide-dependent enzymes with light.
Emmanuel et al. (2016)
5 more

2 mentions without citation

Structural characterization of the thermostable Bradyrhizobium japonicumD-sorbitol dehydrogenase.
Fredslund et al. (2016)
1 more