› 3oxd

X-ray diffraction
3Å resolution

Crystal structure of glycine riboswitch with two mutations

Released:
DOI: 10.2210/pdb3oxd/pdb
PDB entry 3oxd coloured by chain, front view.
PDB entry 3oxd coloured by chain, side view.
PDB entry 3oxd coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structural insights into ligand recognition by a sensing domain of the cooperative glycine riboswitch.
Huang L, Serganov A, Patel DJ
Mol Cell 40 774-86 (2010)
PMID: 21145485

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195889 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
domain II of glycine riboswitch Chain: A
Molecule details ›
Chain: A
Length: 88 nucleotides
Theoretical weight: 28.74 KDa
Source organism: Vibrio cholerae
Expression system: Not provided
Sequence domains: Glycine riboswitch
domain II of glycine riboswitch Chain: B
Molecule details ›
Chain: B
Length: 88 nucleotides
Theoretical weight: 28.66 KDa
Source organism: Vibrio cholerae
Expression system: Not provided
Sequence domains: Glycine riboswitch

Ligands and Environments

1 bound ligand:
Ligand MG 34 x MG
2 modified residues:
Ligand GDP 1 x GDP
Ligand CCC 2 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 83.969Å b: 83.969Å c: 199.998Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.214
Expression system: Not provided

Quick links

Citations

30 review citations

A decade of riboswitches.
Serganov et al. (2013)
29 more

2 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
1 more