› 3obk

X-ray diffraction
2.5Å resolution

Crystal structure of delta-aminolevulinic acid dehydratase (porphobilinogen synthase) from toxoplasma gondii ME49 in complex with the reaction product porphobilinogen

Released:
DOI: 10.2210/pdb3obk/pdb
PDB entry 3obk coloured by chain, front view.
PDB entry 3obk coloured by chain, side view.
PDB entry 3obk coloured by chain, top view.
Source organism: Toxoplasma gondii ME49
Primary publication:
Crystal structure of Toxoplasma gondii porphobilinogen synthase: insights on octameric structure and porphobilinogen formation.
Jaffe EK, Shanmugam D, Gardberg A, Dieterich S, Sankaran B, Stewart LJ, Myler PJ, Roos DS
J Biol Chem 286 15298-307 (2011)
PMID: 21383008

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 356 amino acids
Theoretical weight: 39.54 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand CL 16 x CL
Ligand PBG 8 x PBG
Ligand EDO 19 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 177.11Å b: 187.17Å c: 95.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Dynamic dissociating homo-oligomers and the control of protein function.
Selwood et al. (2012)
4 more

5 mentions without citation

Allostery and the dynamic oligomerization of porphobilinogen synthase.
Jaffe et al. (2012)
4 more