› 3obb

X-ray diffraction
2.2Å resolution

Crystal structure of a possible 3-hydroxyisobutyrate Dehydrogenase from pseudomonas aeruginosa pao1

Released:
DOI: 10.2210/pdb3obb/pdb
PDB entry 3obb coloured by chain, front view.
PDB entry 3obb coloured by chain, side view.
PDB entry 3obb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Biochemical and structural studies of uncharacterized protein PA0743 from Pseudomonas aeruginosa revealed NAD+-dependent L-serine dehydrogenase.
Tchigvintsev A, Singer A, Brown G, Flick R, Evdokimova E, Tan K, Gonzalez CF, Savchenko A, Yakunin AF
J Biol Chem 287 1874-83 (2012)
PMID: 22128181

Function and Biology Details

Reaction catalysed: 
(1a) L-serine + NAD(+) = 2-aminomalonate semialdehyde + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent L-serine dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 31.63 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I5I6 (Residues: 1-298; Coverage: 100%)
Gene name: PA0743
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 3 x ACT
Ligand EDO 1 x EDO
Ligand EPE 1 x EPE
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6222
Unit cell:
a: 92.611Å b: 92.611Å c: 124.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.197 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Double trouble-Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments.
Majorek et al. (2014)
6 more

4 mentions without citation

Biochemical and structural studies of uncharacterized protein PA0743 from Pseudomonas aeruginosa revealed NAD+-dependent L-serine dehydrogenase.
Tchigvintsev et al. (2012)
3 more