3ob1 Summary


Crystal structure of c-Cbl TKB domain in complex with double phosphorylated Spry2 peptide

The structure was published by Sun, Q., Jackson, R.A., Ng, C., Guy, G.R., and Sivaraman, J., in 2010 in a paper entitled "Additional serine/threonine phosphorylation reduces binding affinity but preserves interface topography of substrate proteins to the c-Cbl TKB domain" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 12-meric peptide from Protein sprouty homolog 2 and E3 ubiquitin-protein ligase CBL.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A 12-meric peptide from Protein sprouty homolog 2 O43597 (49-60) (SPY2_HUMAN)search Homo sapienssearch < 90% 12 83%
B E3 ubiquitin-protein ligase CBL P22681 (25-351) (CBL_HUMAN)search Homo sapienssearch < 90% 329 89%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
O43597 (49 - 60) 12-meric peptide from Protein sprouty homolog 2 Homo sapiens
P22681 (25 - 351) E3 ubiquitin-protein ligase CBL Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
B Transcription Elongation Factor S-II; Chain Asearch, EF-handsearch, SHC Adaptor Proteinsearch CBL proto-oncogene N-terminal domain 1search, CBL proto-oncogene N-terminus, EF hand-like domainsearch, CBL proto-oncogene N-terminus, SH2-like domainsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
B (P22681) phosphotyrosine bindingsearch calcium ion bindingsearch signal transducer activitysearch ubiquitin-protein transferase activitysearch nucleussearch cell surface receptor signaling pathwaysearch regulation of signalingsearch

Chain InterPro annotation
B SH2 domainsearch Adaptor protein Cbl, N-terminal helicalsearch EF-hand domain pairsearch Adaptor protein Cbl, EF hand-likesearch Adaptor protein Cbl, SH2-likesearch Adaptor protein Cbl, PTB domainsearch Adaptor protein Cblsearch