› 3oaa

X-ray diffraction
3.26Å resolution

Structure of the E.coli F1-ATP synthase inhibited by subunit Epsilon

Released:
DOI: 10.2210/pdb3oaa/pdb
PDB entry 3oaa coloured by chain, front view.
PDB entry 3oaa coloured by chain, side view.
PDB entry 3oaa coloured by chain, top view.
Source organism: Escherichia coli DH1
Entry authors: Cingolani G, Duncan TM

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141306 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATP synthase subunit alpha Chains: A, B, C, I, J, K, Q, R, S, Y, Z, a
Molecule details ›
Chains: A, B, C, I, J, K, Q, R, S, Y, Z, a
Length: 513 amino acids
Theoretical weight: 55.28 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABB0 (Residues: 1-513; Coverage: 100%)
Gene names: JW3712, atpA, b3734, papA, uncA
Sequence domains:
Structure domains:
ATP synthase subunit beta Chains: D, E, F, L, M, N, T, U, V, b, c, d
Molecule details ›
Chains: D, E, F, L, M, N, T, U, V, b, c, d
Length: 459 amino acids
Theoretical weight: 50.25 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABB4 (Residues: 2-460; Coverage: 100%)
Gene names: JW3710, atpD, b3732, papB, uncD
Sequence domains:
Structure domains:
ATP synthase gamma chain Chains: G, O, W, e
Molecule details ›
Chains: G, O, W, e
Length: 286 amino acids
Theoretical weight: 31.49 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABA6 (Residues: 2-287; Coverage: 100%)
Gene names: JW3711, atpG, b3733, papC, uncG
Sequence domains: ATP synthase
Structure domains:
ATP synthase epsilon chain Chains: H, P, X, f
Molecule details ›
Chains: H, P, X, f
Length: 138 amino acids
Theoretical weight: 14.96 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6E6 (Residues: 2-139; Coverage: 99%)
Gene names: JW3709, atpC, b3731, papG, uncC
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ANP 12 x ANP
Ligand MG 16 x MG
Ligand ADP 4 x ADP
Ligand SO4 17 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, NSLS BEAMLINE X6A, CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 435.97Å b: 183Å c: 225.39Å
α: 90° β: 108.99° γ: 90°
R-values:
R R work R free
0.243 0.243 0.265
Expression system: Escherichia coli

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