3o7l Summary

pdbe.org/3o7l
spacer

Crystal Structure of phospholamban (1-19):PKA C-subunit:AMP-PNP:Mg2+ complex

The structure was published by Masterson, L.R., Cheng, C., Yu, T., et al., Kornev, A., Taylor, S.S., and Veglia, G., in 2010 in a paper entitled "Dynamics connect substrate recognition to catalysis in protein kinase A." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.8 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely cAMP-dependent protein kinase catalytic subunit alpha and Cardiac phospholamban.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
B cAMP-dependent protein kinase catalytic subunit alpha P05132 (2-351) (KAPCA_MOUSE)search Mus musculussearch 96% 350 93%
D cAMP-dependent protein kinase catalytic subunit alpha P05132 (2-351) (KAPCA_MOUSE)search Mus musculussearch 96% 350 95%
I Cardiac phospholamban P61014 (4-18) (PPLA_MOUSE)search Mus musculussearch < 90% 15 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05132 (2 - 351) cAMP-dependent protein kinase catalytic subunit alpha Mus musculus
P61014 (4 - 18) Cardiac phospholamban

Chain Structural classification (CATH) Sequence family (Pfam)
B (P05132) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
D (P05132) PF00069: Protein kinase domainsearch
I (P61014) PF04272: Phospholambansearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
B (P05132) protein kinase activitysearch protein serine/threonine kinase activitysearch protein kinase A regulatory subunit bindingsearch ATP bindingsearch protein kinase bindingsearch protein serine/threonine/tyrosine kinase activitysearch cAMP-dependent protein kinase activitysearch protein bindingsearch kinase activitysearch ubiquitin protein ligase bindingsearch nucleotide bindingsearch transferase activitysearch transferase activity, transferring phosphorus-containing groupssearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch protein phosphorylationsearch protein autophosphorylationsearch regulation of synaptic transmissionsearch mesoderm formationsearch cellular response to parathyroid hormone stimulussearch positive regulation of cell cycle arrestsearch peptidyl-threonine phosphorylationsearch sperm capacitationsearch cellular response to glucose stimulussearch peptidyl-serine phosphorylationsearch regulation of tight junction assemblysearch regulation of protein processingsearch neural tube closuresearch regulation of proteasomal protein catabolic processsearch positive regulation of protein export from nucleussearch phosphorylationsearch regulation of osteoblast differentiationsearch cytoplasmsearch motile ciliumsearch mitochondrionsearch neuromuscular junctionsearch sperm midpiecesearch nucleussearch ciliumsearch AMP-activated protein kinase complexsearch cytosolsearch ciliary basesearch extracellular vesicular exosomesearch membranesearch centrosomesearch plasma membranesearch nucleoplasmsearch cell projectionsearch
D (P05132) ATP bindingsearch protein serine/threonine/tyrosine kinase activitysearch nucleotide bindingsearch protein kinase A regulatory subunit bindingsearch ubiquitin protein ligase bindingsearch protein serine/threonine kinase activitysearch protein bindingsearch cAMP-dependent protein kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch transferase activitysearch protein kinase activitysearch kinase activitysearch protein kinase bindingsearch phosphorylationsearch protein phosphorylationsearch regulation of tight junction assemblysearch cellular response to glucose stimulussearch sperm capacitationsearch positive regulation of cell cycle arrestsearch peptidyl-serine phosphorylationsearch regulation of proteasomal protein catabolic processsearch peptidyl-threonine phosphorylationsearch cellular response to parathyroid hormone stimulussearch regulation of synaptic transmissionsearch mesoderm formationsearch positive regulation of protein export from nucleussearch regulation of osteoblast differentiationsearch protein autophosphorylationsearch regulation of protein processingsearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch neural tube closuresearch mitochondrionsearch neuromuscular junctionsearch membranesearch nucleussearch nucleoplasmsearch cytoplasmsearch sperm midpiecesearch centrosomesearch motile ciliumsearch cell projectionsearch plasma membranesearch extracellular vesicular exosomesearch ciliary basesearch AMP-activated protein kinase complexsearch cytosolsearch ciliumsearch

Chain InterPro annotation
B Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
D Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
I Phospholambansearch