› 3o71

X-ray diffraction
1.95Å resolution

Crystal structure of ERK2/DCC peptide complex

Released:
DOI: 10.2210/pdb3o71/pdb
PDB entry 3o71 coloured by chain, front view.
PDB entry 3o71 coloured by chain, side view.
PDB entry 3o71 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Phosphorylation of DCC by ERK2 is facilitated by direct docking of the receptor P1 domain to the kinase.
Ma W, Shang Y, Wei Z, Wen W, Wang W, Zhang M
Structure 18 1502-11 (2010)
PMID: 21070949

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 41.33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63086 (Residues: 1-358; Coverage: 100%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:
Netrin receptor DCC Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q63155 (Residues: 1140-1166; Coverage: 2%)
Gene name: Dcc

Ligands and Environments

1 bound ligand:
Ligand SCN 2 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P321
Unit cell:
a: 87.178Å b: 87.178Å c: 94.459Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.24
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Revisiting Netrin-1: One Who Guides (Axons).
Boyer et al. (2018)
1 more

7 mentions without citation

Molecular basis of MAP kinase regulation.
Peti et al. (2013)
6 more