› 3o2r

X-ray diffraction
1.25Å resolution

Structural flexibility in region involved in dimer formation of nuclease domain of Ribonuclase III (rnc) from Campylobacter jejuni

Released:
DOI: 10.2210/pdb3o2r/pdb
PDB entry 3o2r coloured by chain, front view.
PDB entry 3o2r coloured by chain, side view.
PDB entry 3o2r coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Minasov G, Halavaty A, Shuvalova L, Dubrovska I, Winsor J, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192912 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 170 amino acids
Theoretical weight: 19.14 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PM40 (Residues: 1-146; Coverage: 65%)
Gene names: Cj1635c, rnc
Sequence domains: Ribonuclease-III-like
Structure domains: Ribonuclease III domain
Ribonuclease 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 170 amino acids
Theoretical weight: 19.15 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PM40 (Residues: 1-146; Coverage: 65%)
Gene names: Cj1635c, rnc
Sequence domains: Ribonuclease-III-like
Structure domains: Ribonuclease III domain

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
1 modified residue:
Ligand MLZ 2 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 38.451Å b: 61.552Å c: 118.093Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.135 0.133 0.168
Expression system: Escherichia coli BL21(DE3)

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