› 3nxs

X-ray diffraction
2.3Å resolution

Crystal structure of LAO/AO transport system from Mycobacterium smegmatis bound to GDP

Released:
DOI: 10.2210/pdb3nxs/pdb
PDB entry 3nxs coloured by chain, front view.
PDB entry 3nxs coloured by chain, side view.
PDB entry 3nxs coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Edwards TE, Baugh L, Bullen J, Baydo RO, Witte P, Thompkins K, Phan IQ, Abendroth J, Clifton MC, Sankaran B, Van Voorhis WC, Myler PJ, Staker BL, Grundner C, Lorimer DD
J Struct Funct Genomics 16 91-9 (2015)
PMID: 25832174

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA+ ATPase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 35.53 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QX37 (Residues: 1-325; Coverage: 100%)
Gene name: MSMEG_3160
Sequence domains: Methylmalonyl Co-A mutase-associated GTPase MeaB
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 78.63Å b: 91.46Å c: 57.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.231
Expression system: Escherichia coli

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Citations

2 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
1 more

2 mentions without citation

Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Edwards et al. (2015)
1 more