› 3ntt

X-ray diffraction
3.45Å resolution

Structural insights of Adeno-Associated virus 5. A gene therapy Vector for Cystic Fibrosis

Released:
DOI: 10.2210/pdb3ntt/pdb
PDB entry 3ntt coloured by chain, front view.
PDB entry 3ntt coloured by chain, side view.
PDB entry 3ntt coloured by chain, top view.
Source organism: adeno-associated virus 5
Primary publication:
Structural insights into adeno-associated virus serotype 5.
Govindasamy L, DiMattia MA, Gurda BL, Halder S, McKenna R, Chiorini JA, Muzyczka N, Zolotukhin S, Agbandje-McKenna M
J Virol 87 11187-99 (2013)
PMID: 23926356

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 60-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein Chain: A
Molecule details ›
Chain: A
Length: 724 amino acids
Theoretical weight: 80.5 KDa
Source organism: adeno-associated virus 5
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9YIJ1 (Residues: 1-724; Coverage: 100%)
Gene names: VP1, cap
Sequence domains:
Structure domains: Parvovirus coat protein VP1/VP2

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 264.7Å b: 447.9Å c: 629.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.252
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

Engineering adeno-associated viruses for clinical gene therapy.
Kotterman et al. (2014)
9 more

17 mentions without citation

Adeno-Associated Virus (AAV) Gene Delivery: Dissecting Molecular Interactions upon Cell Entry.
Large et al. (2021)
16 more