› 3nrx

X-ray diffraction
1.75Å resolution

Insights into anti-parallel microtubule crosslinking by PRC1, a conserved non-motor microtubule binding protein

Released:
DOI: 10.2210/pdb3nrx/pdb
PDB entry 3nrx coloured by chain, front view.
PDB entry 3nrx coloured by chain, side view.
PDB entry 3nrx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into antiparallel microtubule crosslinking by PRC1, a conserved nonmotor microtubule binding protein.
Subramanian R, Wilson-Kubalek EM, Arthur CP, Bick MJ, Campbell EA, Darst SA, Milligan RA, Kapoor TM
Cell 142 433-43 (2010)
PMID: 20691902

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein regulator of cytokinesis 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43663 (Residues: 341-466; Coverage: 20%)
Gene name: PRC1
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P32
Unit cell:
a: 49.078Å b: 49.078Å c: 94.784Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.198 0.269
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Molecular control of animal cell cytokinesis.
Fededa et al. (2012)
25 more

4 mentions without citation

Challenges and opportunities in the high-resolution cryo-EM visualization of microtubules and their binding partners.
Nogales et al. (2017)
3 more