› 3nov

X-ray diffraction
1.05Å resolution

Crystal Structure of D17E Isocyanide Hydratase from Pseudomonas fluorescens

Released:
DOI: 10.2210/pdb3nov/pdb
PDB entry 3nov coloured by chain, front view.
PDB entry 3nov coloured by chain, side view.
PDB entry 3nov coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.
Lakshminarasimhan M, Madzelan P, Nan R, Milkovic NM, Wilson MA
J Biol Chem 285 29651-61 (2010)
PMID: 20630867

Function and Biology Details

Reaction catalysed: 
N-cyclohexylformamide = cyclohexyl isocyanide + H(2)O
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DJ-1/PfpI domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 24.19 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4K977 (Residues: 1-228; Coverage: 100%)
Gene names: PFL_4109, inhA
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: I212121
Unit cell:
a: 38.699Å b: 102.569Å c: 121.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.174
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Protein Promiscuity in H2O2 Signaling.
Young et al. (2019)
1 more

1 mention without citation

Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.
Lakshminarasimhan et al. (2010)