› 3noq

X-ray diffraction
1Å resolution

Crystal Structure of C101S Isocyanide Hydratase from Pseudomonas fluorescens

Released:
DOI: 10.2210/pdb3noq/pdb
PDB entry 3noq coloured by chain, front view.
PDB entry 3noq coloured by chain, side view.
PDB entry 3noq coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.
Lakshminarasimhan M, Madzelan P, Nan R, Milkovic NM, Wilson MA
J Biol Chem 285 29651-61 (2010)
PMID: 20630867

Function and Biology Details

Reaction catalysed: 
N-cyclohexylformamide = cyclohexyl isocyanide + H(2)O
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DJ-1/PfpI domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 24.16 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4K977 (Residues: 1-228; Coverage: 100%)
Gene names: PFL_4109, inhA
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand NHE 2 x NHE
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 56.313Å b: 56.403Å c: 68.296Å
α: 90° β: 112.42° γ: 90°
R-values:
R R work R free
0.125 0.124 0.146
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Protein Promiscuity in H2O2 Signaling.
Young et al. (2019)
1 more

4 mentions without citation

New tools for the analysis and validation of cryo-EM maps and atomic models.
Afonine et al. (2018)
3 more