› 3njp

X-ray diffraction
3.3Å resolution

The Extracellular and Transmembrane Domain Interfaces in Epidermal Growth Factor Receptor Signaling

Released:
DOI: 10.2210/pdb3njp/pdb
PDB entry 3njp coloured by chain, front view.
PDB entry 3njp coloured by chain, side view.
PDB entry 3njp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural evidence for loose linkage between ligand binding and kinase activation in the epidermal growth factor receptor.
Lu C, Mi LZ, Grey MJ, Zhu J, Graef E, Yokoyama S, Springer TA
Mol Cell Biol 30 5432-43 (2010)
PMID: 20837704

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132672 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 67.99 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00533 (Residues: 25-638; Coverage: 52%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Structure domains:
Epidermal growth factor Chains: C, D
Molecule details ›
Chains: C, D
Length: 47 amino acids
Theoretical weight: 5.56 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01133 (Residues: 975-1021; Coverage: 4%)
Gene name: EGF
Sequence domains: EGF-like domain
Structure domains: Laminin

Ligands and Environments

2 bound ligands:
Ligand NAG 12 x NAG
Ligand 2PE 5 x 2PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P3121
Unit cell:
a: 220.167Å b: 220.167Å c: 113.123Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.263 0.298
Expression system: Cricetulus griseus

Quick links

Citations

15 review citations

The ErbB/HER family of protein-tyrosine kinases and cancer.
Roskoski R. (2014)
14 more

72 mentions without citation

A structural perspective on the regulation of the epidermal growth factor receptor.
Kovacs et al. (2015)
71 more