› 3nhn

X-ray diffraction
2.61Å resolution

Crystal structure of the SRC-family kinase HCK SH3-SH2-linker regulatory region

Released:
DOI: 10.2210/pdb3nhn/pdb
PDB entry 3nhn coloured by chain, front view.
PDB entry 3nhn coloured by chain, side view.
PDB entry 3nhn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Src family kinase Hck SH3-SH2 linker regulatory region supports an SH3-dominant activation mechanism.
Alvarado JJ, Betts L, Moroco JA, Smithgall TE, Yeh JI
J Biol Chem 285 35455-61 (2010)
PMID: 20810664

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase HCK Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08631 (Residues: 72-256; Coverage: 35%)
Gene name: HCK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P31
Unit cell:
a: 60.714Å b: 60.714Å c: 49.581Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.12 0.119 0.176
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Polyproline-II helix in proteins: structure and function.
Adzhubei et al. (2013)
3 more

6 mentions without citation

Unraveling the molecular architecture of a G protein-coupled receptor/β-arrestin/Erk module complex.
Bourquard et al. (2015)
5 more