› 3ngd

X-ray diffraction
2.8Å resolution

Structural Basis for Proficient Incorporation of dTTP Opposite O6-methylguanine by Human DNA Polymerase Iota

Released:
DOI: 10.2210/pdb3ngd/pdb
PDB entry 3ngd coloured by chain, front view.
PDB entry 3ngd coloured by chain, side view.
PDB entry 3ngd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for proficient incorporation of dTTP opposite O6-methylguanine by human DNA polymerase iota.
Pence MG, Choi JY, Egli M, Guengerich FP
J Biol Chem 285 40666-72 (2010)
PMID: 20961860

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116615 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA polymerase iota Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 47.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNA4 (Residues: 26-445; Coverage: 57%)
Gene names: POLI, RAD30B
Sequence domains:
Structure domains:
5'-D(*TP*CP*TP*(6OG)P*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*(DOC))-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand DCP 1 x DCP
2 modified residues:
Ligand 6OG 2 x 6OG
Ligand DOC 2 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P6522
Unit cell:
a: 97.999Å b: 97.999Å c: 202.552Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Multifaceted roles of alkyltransferase and related proteins in DNA repair, DNA damage, resistance to chemotherapy, and research tools.
Pegg AE. (2011)
2 more

2 mentions without citation

Structural basis for proficient incorporation of dTTP opposite O6-methylguanine by human DNA polymerase iota.
Pence et al. (2010)
1 more