› 3ncr

X-ray diffraction
1.44Å resolution

GlnK2 from Archaeoglubus fulgidus, ADP complex

Released:
DOI: 10.2210/pdb3ncr/pdb
PDB entry 3ncr coloured by chain, front view.
PDB entry 3ncr coloured by chain, side view.
PDB entry 3ncr coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Cooperative binding of MgATP and MgADP in the trimeric P(II) protein GlnK2 from Archaeoglobus fulgidus.
Helfmann S, Lü W, Litz C, Andrade SL
J Mol Biol 402 165-77 (2010)
PMID: 20643148

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-128036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein GlnK2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 119 amino acids
Theoretical weight: 13.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28527 (Residues: 1-112; Coverage: 100%)
Gene names: AF_1747, glnK2
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

5 bound ligands:
Ligand ADP 3 x ADP
Ligand CL 1 x CL
Ligand PO4 4 x PO4
Ligand MG 3 x MG
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: I422
Unit cell:
a: 123.83Å b: 123.83Å c: 125.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

P(II) signal transduction proteins: nitrogen regulation and beyond.
Huergo et al. (2013)
3 more

1 mention without citation

Structural basis and target-specific modulation of ADP sensing by the Synechococcus elongatus PII signaling protein.
Zeth et al. (2014)