3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Sequence family (Pfam)
A, B (P05108) PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P05108) heme bindingsearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch iron ion bindingsearch cholesterol monooxygenase (side-chain-cleaving) activitysearch oxidoreductase activitysearch metal ion bindingsearch monooxygenase activitysearch cholesterol bindingsearch oxidation-reduction processsearch C21-steroid hormone biosynthetic processsearch response to estrogensearch Schwann cell differentiationsearch response to corticosteronesearch response to organic cyclic compoundsearch cholesterol metabolic processsearch cellular response to antibioticsearch response to gamma radiationsearch cellular response to cAMPsearch response to organic substancesearch response to cadmium ionsearch hippocampus developmentsearch steroid biosynthetic processsearch response to hydrogen peroxidesearch response to alkaloidsearch progesterone biosynthetic processsearch phenol-containing compound metabolic processsearch response to antibioticsearch response to drugsearch phthalate metabolic processsearch maternal process involved in female pregnancysearch response to steroid hormonesearch xenobiotic metabolic processsearch response to ionizing radiationsearch cellular response to tumor necrosis factorsearch response to vitamin Esearch fractalkine metabolic processsearch response to acidsearch response to genisteinsearch response to insecticidesearch cellular response to follicle-stimulating hormone stimulussearch steroid metabolic processsearch dibenzo-p-dioxin metabolic processsearch biphenyl metabolic processsearch lipid metabolic processsearch response to L-ascorbic acidsearch Leydig cell differentiationsearch response to fungicidesearch cellular response to peptide hormone stimulussearch response to cAMPsearch response to peptide hormonesearch cellular response to gonadotropin stimulussearch estrogen biosynthetic processsearch C21-steroid hormone metabolic processsearch sterol metabolic processsearch organic acid metabolic processsearch male gonad developmentsearch vitamin D metabolic processsearch response to gonadotropinsearch cellular response to lipopolysaccharidesearch granulosa cell differentiationsearch small molecule metabolic processsearch testosterone biosynthetic processsearch mating behaviorsearch cellular response to fibroblast growth factor stimulussearch cellular response to transforming growth factor beta stimulussearch cellular response to interleukin-1search response to nutrientsearch response to salt stresssearch cerebellum developmentsearch cellular response to cadmium ionsearch mitochondrial matrixsearch mitochondrionsearch mitochondrial cristasearch membranesearch perikaryonsearch mitochondrial inner membranesearch mitochondrial membranesearch
C, D (P10109) electron carrier activitysearch iron-sulfur cluster bindingsearch 2 iron, 2 sulfur cluster bindingsearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch