3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Sequence family (Pfam)
A, B (P05108) PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P05108) cellular response to interleukin-1search organic acid metabolic processsearch response to gonadotropinsearch steroid metabolic processsearch phenol-containing compound metabolic processsearch steroid biosynthetic processsearch C21-steroid hormone biosynthetic processsearch small molecule metabolic processsearch response to antibioticsearch response to alkaloidsearch cellular response to fibroblast growth factor stimulussearch maternal process involved in female pregnancysearch C21-steroid hormone metabolic processsearch Leydig cell differentiationsearch response to peptide hormonesearch cholesterol metabolic processsearch oxidation-reduction processsearch phthalate metabolic processsearch cellular response to follicle-stimulating hormone stimulussearch response to estrogensearch response to acid chemicalsearch lipid metabolic processsearch cellular response to antibioticsearch response to nutrientsearch response to ionizing radiationsearch response to insecticidesearch cellular response to transforming growth factor beta stimulussearch response to corticosteronesearch testosterone biosynthetic processsearch cellular response to cAMPsearch response to cadmium ionsearch cellular response to gonadotropin stimulussearch response to organic cyclic compoundsearch cellular response to peptide hormone stimulussearch xenobiotic metabolic processsearch response to L-ascorbic acidsearch response to genisteinsearch response to fungicidesearch biphenyl metabolic processsearch hippocampus developmentsearch response to cAMPsearch vitamin D metabolic processsearch sterol metabolic processsearch fractalkine metabolic processsearch cerebellum developmentsearch estrogen biosynthetic processsearch progesterone biosynthetic processsearch response to gamma radiationsearch response to vitamin Esearch dibenzo-p-dioxin metabolic processsearch granulosa cell differentiationsearch mating behaviorsearch response to salt stresssearch Schwann cell differentiationsearch response to drugsearch cellular response to lipopolysaccharidesearch cellular response to tumor necrosis factorsearch response to hydrogen peroxidesearch male gonad developmentsearch response to steroid hormonesearch response to organic substancesearch cellular response to cadmium ionsearch metal ion bindingsearch heme bindingsearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch iron ion bindingsearch oxidoreductase activitysearch cholesterol monooxygenase (side-chain-cleaving) activitysearch monooxygenase activitysearch cholesterol bindingsearch mitochondrial matrixsearch mitochondrial membranesearch membranesearch mitochondrionsearch perikaryonsearch mitochondrial cristasearch mitochondrial inner membranesearch
C, D (P10109) 2 iron, 2 sulfur cluster bindingsearch iron-sulfur cluster bindingsearch electron carrier activitysearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch