3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P05108) Cytochrome p450search PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P05108) oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch heme bindingsearch iron ion bindingsearch cholesterol bindingsearch cholesterol monooxygenase (side-chain-cleaving) activitysearch monooxygenase activitysearch metal ion bindingsearch oxidoreductase activitysearch oxidation-reduction processsearch steroid metabolic processsearch sterol metabolic processsearch dibenzo-p-dioxin metabolic processsearch response to corticosteronesearch male gonad developmentsearch C21-steroid hormone biosynthetic processsearch hippocampus developmentsearch cerebellum developmentsearch cellular response to follicle-stimulating hormone stimulussearch cholesterol metabolic processsearch phthalate metabolic processsearch response to cAMPsearch response to vitamin Esearch testosterone biosynthetic processsearch cellular response to antibioticsearch response to fungicidesearch organic acid metabolic processsearch xenobiotic metabolic processsearch response to hydrogen peroxidesearch cellular response to gonadotropin stimulussearch Leydig cell differentiationsearch granulosa cell differentiationsearch cellular response to cAMPsearch cellular response to fibroblast growth factor stimulussearch response to ionizing radiationsearch maternal process involved in female pregnancysearch small molecule metabolic processsearch response to gonadotropinsearch mating behaviorsearch response to insecticidesearch C21-steroid hormone metabolic processsearch vitamin D metabolic processsearch response to acid chemicalsearch response to alkaloidsearch cellular response to lipopolysaccharidesearch response to cadmium ionsearch response to nutrientsearch response to organic substancesearch response to genisteinsearch cellular response to tumor necrosis factorsearch response to drugsearch steroid biosynthetic processsearch Schwann cell differentiationsearch response to peptide hormonesearch response to salt stresssearch response to estrogensearch progesterone biosynthetic processsearch biphenyl metabolic processsearch response to steroid hormonesearch response to antibioticsearch lipid metabolic processsearch phenol-containing compound metabolic processsearch response to organic cyclic compoundsearch cellular response to cadmium ionsearch cellular response to peptide hormone stimulussearch cellular response to transforming growth factor beta stimulussearch estrogen biosynthetic processsearch fractalkine metabolic processsearch cellular response to interleukin-1search response to L-ascorbic acidsearch response to gamma radiationsearch mitochondrial matrixsearch mitochondrionsearch mitochondrial membranesearch membranesearch perikaryonsearch mitochondrial inner membranesearch mitochondrial cristasearch
C, D (P10109) iron-sulfur cluster bindingsearch electron carrier activitysearch 2 iron, 2 sulfur cluster bindingsearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch