3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P05108) Cytochrome p450search PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A, B (P05108) oxidation-reduction processsearch hippocampus developmentsearch cellular response to lipopolysaccharidesearch C21-steroid hormone metabolic processsearch cholesterol metabolic processsearch response to vitamin Esearch cellular response to cadmium ionsearch response to estrogensearch Schwann cell differentiationsearch fractalkine metabolic processsearch C21-steroid hormone biosynthetic processsearch cellular response to antibioticsearch steroid biosynthetic processsearch response to genisteinsearch lipid metabolic processsearch biphenyl metabolic processsearch cellular response to follicle-stimulating hormone stimulussearch response to organic substancesearch sterol metabolic processsearch cellular response to peptide hormone stimulussearch response to cAMPsearch response to fungicidesearch male gonad developmentsearch cellular response to cAMPsearch phthalate metabolic processsearch response to salt stresssearch testosterone biosynthetic processsearch response to corticosteronesearch response to acid chemicalsearch xenobiotic metabolic processsearch progesterone biosynthetic processsearch estrogen biosynthetic processsearch response to drugsearch response to insecticidesearch steroid metabolic processsearch response to hydrogen peroxidesearch cellular response to gonadotropin stimulussearch Leydig cell differentiationsearch vitamin D metabolic processsearch response to gamma radiationsearch cellular response to transforming growth factor beta stimulussearch dibenzo-p-dioxin metabolic processsearch response to alkaloidsearch response to cadmium ionsearch response to L-ascorbic acidsearch response to nutrientsearch organic acid metabolic processsearch mating behaviorsearch response to antibioticsearch small molecule metabolic processsearch response to steroid hormonesearch response to ionizing radiationsearch response to gonadotropinsearch granulosa cell differentiationsearch maternal process involved in female pregnancysearch cellular response to interleukin-1search cerebellum developmentsearch phenol-containing compound metabolic processsearch cellular response to tumor necrosis factorsearch response to organic cyclic compoundsearch response to peptide hormonesearch cellular response to fibroblast growth factor stimulussearch mitochondrial matrixsearch mitochondrial inner membranesearch mitochondrionsearch perikaryonsearch mitochondrial cristasearch membranesearch mitochondrial membranesearch cholesterol monooxygenase (side-chain-cleaving) activitysearch oxidoreductase activitysearch cholesterol bindingsearch heme bindingsearch metal ion bindingsearch monooxygenase activitysearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch iron ion bindingsearch
C, D (P10109) electron carrier activitysearch 2 iron, 2 sulfur cluster bindingsearch iron-sulfur cluster bindingsearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch