3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P05108) Cytochrome p450search PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P05108) oxidation-reduction processsearch phenol-containing compound metabolic processsearch cholesterol metabolic processsearch cellular response to follicle-stimulating hormone stimulussearch C21-steroid hormone biosynthetic processsearch maternal process involved in female pregnancysearch cellular response to fibroblast growth factor stimulussearch cerebellum developmentsearch response to L-ascorbic acidsearch hippocampus developmentsearch response to cAMPsearch response to acid chemicalsearch estrogen biosynthetic processsearch organic acid metabolic processsearch biphenyl metabolic processsearch C21-steroid hormone metabolic processsearch Schwann cell differentiationsearch response to nutrientsearch xenobiotic metabolic processsearch response to corticosteronesearch phthalate metabolic processsearch response to vitamin Esearch cellular response to gonadotropin stimulussearch response to steroid hormonesearch steroid biosynthetic processsearch granulosa cell differentiationsearch response to estrogensearch response to gonadotropinsearch response to gamma radiationsearch response to ionizing radiationsearch sterol metabolic processsearch cellular response to tumor necrosis factorsearch cellular response to interleukin-1search response to organic substancesearch cellular response to lipopolysaccharidesearch lipid metabolic processsearch response to antibioticsearch dibenzo-p-dioxin metabolic processsearch progesterone biosynthetic processsearch fractalkine metabolic processsearch cellular response to peptide hormone stimulussearch steroid metabolic processsearch Leydig cell differentiationsearch vitamin D metabolic processsearch response to alkaloidsearch response to organic cyclic compoundsearch response to hydrogen peroxidesearch male gonad developmentsearch response to fungicidesearch response to insecticidesearch cellular response to cAMPsearch mating behaviorsearch cellular response to antibioticsearch response to peptide hormonesearch response to salt stresssearch response to genisteinsearch small molecule metabolic processsearch response to cadmium ionsearch cellular response to cadmium ionsearch response to drugsearch cellular response to transforming growth factor beta stimulussearch testosterone biosynthetic processsearch heme bindingsearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch iron ion bindingsearch oxidoreductase activitysearch cholesterol monooxygenase (side-chain-cleaving) activitysearch metal ion bindingsearch cholesterol bindingsearch monooxygenase activitysearch mitochondrial matrixsearch mitochondrionsearch membranesearch mitochondrial cristasearch mitochondrial inner membranesearch perikaryonsearch mitochondrial membranesearch
C, D (P10109) electron carrier activitysearch iron-sulfur cluster bindingsearch 2 iron, 2 sulfur cluster bindingsearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch