3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P05108) Cytochrome p450search PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P05108) oxidation-reduction processsearch granulosa cell differentiationsearch C21-steroid hormone metabolic processsearch response to organic substancesearch cholesterol metabolic processsearch response to cadmium ionsearch C21-steroid hormone biosynthetic processsearch steroid metabolic processsearch organic acid metabolic processsearch response to ionizing radiationsearch cellular response to tumor necrosis factorsearch cellular response to transforming growth factor beta stimulussearch steroid biosynthetic processsearch cerebellum developmentsearch male gonad developmentsearch cellular response to gonadotropin stimulussearch cellular response to follicle-stimulating hormone stimulussearch testosterone biosynthetic processsearch progesterone biosynthetic processsearch response to cAMPsearch biphenyl metabolic processsearch cellular response to cAMPsearch sterol metabolic processsearch response to gamma radiationsearch estrogen biosynthetic processsearch response to hydrogen peroxidesearch response to antibioticsearch cellular response to peptide hormone stimulussearch response to insecticidesearch response to vitamin Esearch response to L-ascorbic acidsearch phthalate metabolic processsearch vitamin D metabolic processsearch Leydig cell differentiationsearch xenobiotic metabolic processsearch response to drugsearch response to gonadotropinsearch fractalkine metabolic processsearch response to genisteinsearch response to acid chemicalsearch response to alkaloidsearch cellular response to cadmium ionsearch lipid metabolic processsearch response to organic cyclic compoundsearch cellular response to antibioticsearch response to salt stresssearch response to steroid hormonesearch response to corticosteronesearch maternal process involved in female pregnancysearch response to nutrientsearch small molecule metabolic processsearch cellular response to interleukin-1search Schwann cell differentiationsearch response to estrogensearch response to peptide hormonesearch phenol-containing compound metabolic processsearch response to fungicidesearch mating behaviorsearch cellular response to lipopolysaccharidesearch dibenzo-p-dioxin metabolic processsearch hippocampus developmentsearch cellular response to fibroblast growth factor stimulussearch heme bindingsearch cholesterol monooxygenase (side-chain-cleaving) activitysearch oxidoreductase activitysearch cholesterol bindingsearch metal ion bindingsearch monooxygenase activitysearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch iron ion bindingsearch mitochondrial matrixsearch membranesearch mitochondrionsearch perikaryonsearch mitochondrial cristasearch mitochondrial inner membranesearch mitochondrial membranesearch
C, D (P10109) iron-sulfur cluster bindingsearch 2 iron, 2 sulfur cluster bindingsearch electron carrier activitysearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch