3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P05108) Cytochrome p450search PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B (P05108) oxidation-reduction processsearch cellular response to antibioticsearch cholesterol metabolic processsearch response to vitamin Esearch cellular response to follicle-stimulating hormone stimulussearch C21-steroid hormone biosynthetic processsearch cellular response to gonadotropin stimulussearch cellular response to interleukin-1search response to organic substancesearch response to gonadotropinsearch phenol-containing compound metabolic processsearch cellular response to cadmium ionsearch response to cAMPsearch steroid metabolic processsearch sterol metabolic processsearch testosterone biosynthetic processsearch C21-steroid hormone metabolic processsearch xenobiotic metabolic processsearch response to salt stresssearch hippocampus developmentsearch steroid biosynthetic processsearch response to fungicidesearch dibenzo-p-dioxin metabolic processsearch granulosa cell differentiationsearch response to organic cyclic compoundsearch response to insecticidesearch response to nutrientsearch cellular response to lipopolysaccharidesearch small molecule metabolic processsearch male gonad developmentsearch cellular response to tumor necrosis factorsearch response to genisteinsearch response to antibioticsearch response to corticosteronesearch response to gamma radiationsearch vitamin D metabolic processsearch mating behaviorsearch response to cadmium ionsearch cerebellum developmentsearch response to L-ascorbic acidsearch maternal process involved in female pregnancysearch cellular response to transforming growth factor beta stimulussearch cellular response to cAMPsearch response to ionizing radiationsearch phthalate metabolic processsearch lipid metabolic processsearch response to hydrogen peroxidesearch biphenyl metabolic processsearch response to estrogensearch cellular response to peptide hormone stimulussearch progesterone biosynthetic processsearch fractalkine metabolic processsearch estrogen biosynthetic processsearch response to drugsearch organic acid metabolic processsearch response to alkaloidsearch response to acid chemicalsearch Leydig cell differentiationsearch Schwann cell differentiationsearch response to peptide hormonesearch cellular response to fibroblast growth factor stimulussearch response to steroid hormonesearch heme bindingsearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch cholesterol monooxygenase (side-chain-cleaving) activitysearch iron ion bindingsearch metal ion bindingsearch oxidoreductase activitysearch cholesterol bindingsearch monooxygenase activitysearch mitochondrial matrixsearch mitochondrial inner membranesearch mitochondrial cristasearch perikaryonsearch mitochondrionsearch membranesearch mitochondrial membranesearch
C, D (P10109) 2 iron, 2 sulfur cluster bindingsearch iron-sulfur cluster bindingsearch electron carrier activitysearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch