3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Sequence family (Pfam)
A, B (P05108) PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P05108) heme bindingsearch electron carrier activitysearch iron ion bindingsearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch cholesterol monooxygenase (side-chain-cleaving) activitysearch metal ion bindingsearch cholesterol bindingsearch oxidoreductase activitysearch monooxygenase activitysearch oxidation-reduction processsearch cellular response to lipopolysaccharidesearch vitamin D metabolic processsearch mating behaviorsearch xenobiotic metabolic processsearch Leydig cell differentiationsearch response to nutrientsearch lipid metabolic processsearch steroid metabolic processsearch response to acidsearch response to gonadotropin stimulussearch biphenyl metabolic processsearch granulosa cell differentiationsearch cellular response to tumor necrosis factorsearch Schwann cell differentiationsearch response to L-ascorbic acidsearch cholesterol metabolic processsearch dibenzo-p-dioxin metabolic processsearch response to genisteinsearch cellular response to gonadotropin stimulussearch sterol metabolic processsearch C21-steroid hormone biosynthetic processsearch cellular response to cadmium ionsearch response to antibioticsearch response to cadmium ionsearch response to organic cyclic compoundsearch progesterone biosynthetic processsearch C21-steroid hormone metabolic processsearch organic acid metabolic processsearch small molecule metabolic processsearch cellular response to antibioticsearch response to steroid hormone stimulussearch steroid biosynthetic processsearch response to cAMPsearch fractalkine metabolic processsearch cellular response to interleukin-1search cellular response to peptide hormone stimulussearch response to drugsearch maternal process involved in female pregnancysearch cerebellum developmentsearch cellular response to follicle-stimulating hormone stimulussearch cellular response to cAMPsearch cellular response to transforming growth factor beta stimulussearch response to organic substancesearch phenol-containing compound metabolic processsearch testosterone biosynthetic processsearch response to corticosterone stimulussearch response to gamma radiationsearch response to hydrogen peroxidesearch response to estrogen stimulussearch response to ionizing radiationsearch response to fungicidesearch response to peptide hormone stimulussearch estrogen biosynthetic processsearch hippocampus developmentsearch male gonad developmentsearch response to alkaloidsearch cellular response to fibroblast growth factor stimulussearch response to vitamin Esearch phthalate metabolic processsearch response to salt stresssearch response to insecticidesearch mitochondrial matrixsearch mitochondrionsearch mitochondrial cristasearch perikaryonsearch mitochondrial inner membranesearch membranesearch mitochondrial membranesearch
C, D (P10109) electron carrier activitysearch 2 iron, 2 sulfur cluster bindingsearch iron-sulfur cluster bindingsearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch