3n9y Summary


Crystal structure of human CYP11A1 in complex with cholesterol

The structure was published by Strushkevich, N., Mackenzie, F., Cherkesova, T., Grabovec, I., Usanov, S., and Park, H.W., in 2011 in a paper entitled "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cholesterol side-chain cleavage enzyme and Adrenodoxin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
B Cholesterol side-chain cleavage enzyme P05108 (41-521) (CP11A_HUMAN)search Homo sapienssearch 90% 487 96%
C Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%
D Adrenodoxin P10109 (62-175) (ADX_HUMAN)search Homo sapienssearch < 90% 114 59%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05108 (41 - 521) Cholesterol side-chain cleavage enzyme Homo sapiens
P10109 (62 - 175) Adrenodoxin Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P05108) Cytochrome p450search PF00067: Cytochrome P450search
C, D 2Fe-2S iron-sulfur cluster binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P05108) iron ion bindingsearch cholesterol monooxygenase (side-chain-cleaving) activitysearch monooxygenase activitysearch oxidoreductase activitysearch metal ion bindingsearch cholesterol bindingsearch heme bindingsearch oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygensearch fractalkine metabolic processsearch cholesterol metabolic processsearch response to alkaloidsearch C21-steroid hormone biosynthetic processsearch response to acid chemicalsearch response to L-ascorbic acidsearch hippocampus developmentsearch response to steroid hormonesearch male gonad developmentsearch maternal process involved in female pregnancysearch response to cAMPsearch cellular response to fibroblast growth factor stimulussearch cellular response to interleukin-1search response to peptide hormonesearch response to organic cyclic compoundsearch steroid biosynthetic processsearch granulosa cell differentiationsearch cellular response to peptide hormone stimulussearch xenobiotic metabolic processsearch response to corticosteronesearch phenol-containing compound metabolic processsearch cellular response to lipopolysaccharidesearch response to fungicidesearch response to ionizing radiationsearch cellular response to gonadotropin stimulussearch C21-steroid hormone metabolic processsearch response to salt stresssearch response to genisteinsearch estrogen biosynthetic processsearch cellular response to antibioticsearch oxidation-reduction processsearch cellular response to transforming growth factor beta stimulussearch response to organic substancesearch mating behaviorsearch sterol metabolic processsearch vitamin D metabolic processsearch response to gonadotropinsearch response to drugsearch response to estrogensearch Schwann cell differentiationsearch dibenzo-p-dioxin metabolic processsearch response to gamma radiationsearch response to nutrientsearch response to cadmium ionsearch cerebellum developmentsearch phthalate metabolic processsearch response to insecticidesearch cellular response to cadmium ionsearch lipid metabolic processsearch response to hydrogen peroxidesearch steroid metabolic processsearch cellular response to tumor necrosis factorsearch Leydig cell differentiationsearch progesterone biosynthetic processsearch cellular response to follicle-stimulating hormone stimulussearch cellular response to cAMPsearch organic acid metabolic processsearch response to antibioticsearch small molecule metabolic processsearch testosterone biosynthetic processsearch biphenyl metabolic processsearch response to vitamin Esearch mitochondrial matrixsearch mitochondrionsearch membranesearch mitochondrial inner membranesearch perikaryonsearch mitochondrial cristasearch
C, D (P10109) iron-sulfur cluster bindingsearch 2 iron, 2 sulfur cluster bindingsearch electron carrier activitysearch

Chain InterPro annotation
A, B Cytochrome P450search Cytochrome P450, E-class, group Isearch Cytochrome P450, conserved sitesearch
C, D 2Fe-2S ferredoxin-type domainsearch Adrenodoxinsearch Beta-grasp domainsearch Adrenodoxin, iron-sulphur binding sitesearch