› 3n6o

X-ray diffraction
2.5Å resolution

Crystal structure of the GEF and P4M domain of DrrA/SidM from Legionella pneumophila

Released:
DOI: 10.2210/pdb3n6o/pdb
PDB entry 3n6o coloured by chain, front view.
PDB entry 3n6o coloured by chain, side view.
PDB entry 3n6o coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Primary publication:
High-affinity binding of phosphatidylinositol 4-phosphate by Legionella pneumophila DrrA.
Schoebel S, Blankenfeldt W, Goody RS, Itzen A
EMBO Rep 11 598-604 (2010)
PMID: 20616805

Function and Biology Details

Reaction catalysed: 
GTP + [protein]-L-tyrosine = diphosphate + [protein]-O(4)-(5'-guanylyl)-L-tyrosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multifunctional virulence effector protein DrrA Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 35.12 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZSQ3 (Residues: 340-647; Coverage: 48%)
Gene names: drrA, lpg2464, sidM
Sequence domains:
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 74.962Å b: 75.375Å c: 130.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
20 more

5 mentions without citation

Conditional peripheral membrane proteins: facing up to limited specificity.
Moravcevic et al. (2012)
4 more