› 3mx8

X-ray diffraction
2.1Å resolution

Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor

Released:
DOI: 10.2210/pdb3mx8/pdb
PDB entry 3mx8 coloured by chain, front view.
PDB entry 3mx8 coloured by chain, side view.
PDB entry 3mx8 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor.
Arnold U, Leich F, Neumann P, Lilie H, Ulbrich-Hofmann R
FEBS J 278 331-40 (2011)
PMID: 21134128

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 27.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 99%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 100.607Å b: 32.667Å c: 73.188Å
α: 90° β: 91.81° γ: 90°
R-values:
R R work R free
0.183 0.179 0.258
Expression system: Escherichia coli

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Citations

2 review citations

Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.
Gotte et al. (2019)
1 more