› 3mop

X-ray diffraction
3.4Å resolution

The ternary Death Domain complex of MyD88, IRAK4, and IRAK2

Released:
DOI: 10.2210/pdb3mop/pdb
PDB entry 3mop coloured by chain, front view.
PDB entry 3mop coloured by chain, side view.
PDB entry 3mop coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR/IL-1R signalling.
Lin SC, Lo YC, Wu H
Nature 465 885-90 (2010)
PMID: 20485341

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-128866 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myeloid differentiation primary response protein MyD88 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 110 amino acids
Theoretical weight: 12.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99836 (Residues: 20-117; Coverage: 33%)
Gene name: MYD88
Sequence domains: Death domain
Structure domains: Death Domain, Fas
Interleukin-1 receptor-associated kinase 4 Chains: G, H, I, J
Molecule details ›
Chains: G, H, I, J
Length: 113 amino acids
Theoretical weight: 12.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWZ3 (Residues: 4-106; Coverage: 22%)
Gene name: IRAK4
Structure domains: Death Domain, Fas
Interleukin-1 receptor-associated kinase-like 2 Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 111 amino acids
Theoretical weight: 13.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43187 (Residues: 2-112; Coverage: 18%)
Gene name: IRAK2
Sequence domains: Death domain
Structure domains: Death Domain, Fas

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 101.709Å b: 307.116Å c: 187.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.261
Expression system: Escherichia coli

Quick links

Citations

232 review citations

Pathogen recognition by the innate immune system.
Kumar et al. (2011)
231 more

40 mentions without citation

Structural studies of NF-κB signaling.
Zheng et al. (2011)
39 more