3moh Summary


The structure of rat cytosolic PEPCK mutant A467G in complex with phosphoglycolate and GDP

The structure was published by Johnson, T.A. and Holyoak, T., in 2010 in a paper entitled "Increasing the conformational entropy of the Omega-loop lid domain in phosphoenolpyruvate carboxykinase impairs catalysis and decreases catalytic fidelity ." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

This PDB entry contains multiple copies of the structure of Phosphoenolpyruvate carboxykinase, cytosolic [GTP].

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Phosphoenolpyruvate carboxykinase, cytosolic [GTP] P07379 (1-622) (PCKGC_RAT)search Rattus norvegicussearch 100% 624 98%
B Phosphoenolpyruvate carboxykinase, cytosolic [GTP] P07379 (1-622) (PCKGC_RAT)search Rattus norvegicussearch 100% 624 98%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P07379 (1 - 622) Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Rattus norvegicus

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P07379) Phosphoenolpyruvate Carboxykinase, domain 1search, Phosphoenolpyruvate Carboxykinase, domain 2search, Phosphoenolpyruvate Carboxykinase; domain 3search PF00821: Phosphoenolpyruvate carboxykinasesearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P07379) GTP bindingsearch GDP bindingsearch phosphoenolpyruvate carboxykinase activitysearch manganese ion bindingsearch carboxylic acid bindingsearch phosphoenolpyruvate carboxykinase (GTP) activitysearch purine nucleotide bindingsearch magnesium ion bindingsearch metal ion bindingsearch carboxy-lyase activitysearch lyase activitysearch nucleotide bindingsearch gluconeogenesissearch response to activitysearch glycerol biosynthetic process from pyruvatesearch internal protein amino acid acetylationsearch response to insulinsearch glucose metabolic processsearch oxaloacetate metabolic processsearch glucose homeostasissearch lipid metabolic processsearch extracellular vesicular exosomesearch cytosolsearch cytoplasmsearch

Chain InterPro annotation
A, B Phosphoenolpyruvate carboxykinase, GTP-utilisingsearch Phosphoenolpyruvate carboxykinase, N-terminalsearch Phosphoenolpyruvate carboxykinase, C-terminalsearch Phosphoenolpyruvate carboxykinase, GTP-utilising, conserved sitesearch