› 3mof

X-ray diffraction
1.75Å resolution

The structure of rat cytosolic PEPCK mutant A467G in complex with oxalate and GTP

Released:
DOI: 10.2210/pdb3mof/pdb
PDB entry 3mof coloured by chain, front view.
PDB entry 3mof coloured by chain, side view.
PDB entry 3mof coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Increasing the conformational entropy of the Omega-loop lid domain in phosphoenolpyruvate carboxykinase impairs catalysis and decreases catalytic fidelity .
Johnson TA, Holyoak T
Biochemistry 49 5176-87 (2010)
PMID: 20476774

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 624 amino acids
Theoretical weight: 69.63 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand GTP 2 x GTP
Ligand MN 4 x MN
Ligand OXL 2 x OXL
Ligand NA 2 x NA
Ligand 1PE 3 x 1PE
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 61.983Å b: 119.601Å c: 87.068Å
α: 90° β: 106.87° γ: 90°
R-values:
R R work R free
0.182 0.179 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The mitochondrial isoform of phosphoenolpyruvate carboxykinase (PEPCK-M) and glucose homeostasis: has it been overlooked?
Stark et al. (2014)
8 other articles

1 mention without citation

Increasing the conformational entropy of the Omega-loop lid domain in phosphoenolpyruvate carboxykinase impairs catalysis and decreases catalytic fidelity .
Johnson et al. (2010)