3mh3 Summary


PDB entry 3mh3 (supersedes 2ptj)

Mutagenesis of p38 MAP kinase establishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-out state

The structure was published by Bukhtiyarova, M., Karpusas, M., Northrop, K., Namboodiri, H.V., and Springman, E.B., in 2007 in a paper entitled "Mutagenesis of p38alpha MAP kinase establishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-OUT state." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 360 residues which is 100% of the natural sequence. Out of 360 residues 334 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (1-360) (MK14_HUMAN)search Homo sapienssearch 97% 360 92%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (1 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (Q16539) skeletal muscle tissue developmentsearch cellular response to ionizing radiationsearch cellular response to lipopolysaccharidesearch regulation of transcription, DNA-templatedsearch osteoclast differentiationsearch response to stresssearch toll-like receptor signaling pathwaysearch mRNA metabolic processsearch 3'-UTR-mediated mRNA stabilizationsearch toll-like receptor 10 signaling pathwaysearch regulation of transcription from RNA polymerase II promotersearch positive regulation of myoblast differentiationsearch toll-like receptor TLR6:TLR2 signaling pathwaysearch lipopolysaccharide-mediated signaling pathwaysearch Ras protein signal transductionsearch stress-induced premature senescencesearch cartilage condensationsearch toll-like receptor 3 signaling pathwaysearch fatty acid oxidationsearch toll-like receptor 4 signaling pathwaysearch stress-activated MAPK cascadesearch MyD88-independent toll-like receptor signaling pathwaysearch gene expressionsearch chemotaxissearch striated muscle cell differentiationsearch MyD88-dependent toll-like receptor signaling pathwaysearch transmembrane receptor protein serine/threonine kinase signaling pathwaysearch positive regulation of protein import into nucleussearch toll-like receptor 5 signaling pathwaysearch activation of MAPK activitysearch glucose metabolic processsearch positive regulation of reactive oxygen species metabolic processsearch negative regulation of canonical Wnt signaling pathwaysearch response to muramyl dipeptidesearch blood coagulationsearch cellular response to vascular endothelial growth factor stimulussearch signal transductionsearch cellular component movementsearch positive regulation of muscle cell differentiationsearch apoptotic processsearch innate immune responsesearch cellular response to DNA damage stimulussearch protein phosphorylationsearch RNA metabolic processsearch toll-like receptor 9 signaling pathwaysearch signal transduction in response to DNA damagesearch positive regulation of transcription from RNA polymerase II promotersearch chondrocyte differentiationsearch cell morphogenesissearch toll-like receptor 2 signaling pathwaysearch DNA damage checkpointsearch positive regulation of myotube differentiationsearch positive regulation of myoblast fusionsearch positive regulation of blood vessel endothelial cell migrationsearch regulation of sequence-specific DNA binding transcription factor activitysearch response to lipopolysaccharidesearch angiogenesissearch positive regulation of erythrocyte differentiationsearch TRIF-dependent toll-like receptor signaling pathwaysearch transcription, DNA-templatedsearch intracellular signal transductionsearch phosphorylationsearch MAPK cascadesearch cell surface receptor signaling pathwaysearch peptidyl-serine phosphorylationsearch vascular endothelial growth factor receptor signaling pathwaysearch muscle cell differentiationsearch toll-like receptor TLR1:TLR2 signaling pathwaysearch p38MAPK cascadesearch neurotrophin TRK receptor signaling pathwaysearch platelet activationsearch MAP kinase activitysearch protein bindingsearch protein kinase activitysearch nucleotide bindingsearch kinase activitysearch MAP kinase kinase activitysearch protein serine/threonine kinase activitysearch transferase activitysearch ATP bindingsearch NFAT protein bindingsearch nucleoplasmsearch cytosolsearch spindle polesearch nucleussearch cellsearch cytoplasmsearch mitochondrionsearch extracellular vesicular exosomesearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch