3mh0 Summary


PDB entry 3mh0 (supersedes 2pkj)

Mutagenesis of p38 MAP Kinase eshtablishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-out state

The structure was published by Bukhtiyarova, M., Karpusas, M., Northrop, K., Namboodiri, H.V., and Springman, E.B., in 2007 in a paper entitled "Mutagenesis of p38alpha MAP kinase establishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-OUT state." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 360 residues which is 100% of the natural sequence. Out of 360 residues 331 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (1-360) (MK14_HUMAN)search Homo sapienssearch 97% 360 92%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (1 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (Q16539) transferase activity, transferring phosphorus-containing groupssearch ATP bindingsearch protein kinase activitysearch MAP kinase activitysearch protein bindingsearch protein serine/threonine kinase activitysearch kinase activitysearch NFAT protein bindingsearch MAP kinase kinase activitysearch nucleotide bindingsearch transferase activitysearch protein phosphorylationsearch toll-like receptor 3 signaling pathwaysearch chemotaxissearch Ras protein signal transductionsearch positive regulation of protein import into nucleussearch p38MAPK cascadesearch fatty acid oxidationsearch stress-induced premature senescencesearch regulation of sequence-specific DNA binding transcription factor activitysearch lipopolysaccharide-mediated signaling pathwaysearch MyD88-independent toll-like receptor signaling pathwaysearch regulation of transcription from RNA polymerase II promotersearch cellular component movementsearch signal transductionsearch signal transduction in response to DNA damagesearch positive regulation of blood vessel endothelial cell migrationsearch myoblast differentiation involved in skeletal muscle regenerationsearch glucose metabolic processsearch response to lipopolysaccharidesearch cartilage condensationsearch MyD88-dependent toll-like receptor signaling pathwaysearch transcription, DNA-templatedsearch positive regulation of muscle cell differentiationsearch chondrocyte differentiationsearch positive regulation of myoblast differentiationsearch TRIF-dependent toll-like receptor signaling pathwaysearch cellular response to ionizing radiationsearch platelet activationsearch neurotrophin TRK receptor signaling pathwaysearch toll-like receptor 9 signaling pathwaysearch toll-like receptor TLR1:TLR2 signaling pathwaysearch regulation of transcription, DNA-templatedsearch toll-like receptor 4 signaling pathwaysearch toll-like receptor 10 signaling pathwaysearch angiogenesissearch innate immune responsesearch transmembrane receptor protein serine/threonine kinase signaling pathwaysearch cellular response to vascular endothelial growth factor stimulussearch negative regulation of canonical Wnt signaling pathwaysearch blood coagulationsearch positive regulation of erythrocyte differentiationsearch DNA damage checkpointsearch positive regulation of myoblast fusionsearch vascular endothelial growth factor receptor signaling pathwaysearch mRNA metabolic processsearch intracellular signal transductionsearch striated muscle cell differentiationsearch 3'-UTR-mediated mRNA stabilizationsearch response to stresssearch skeletal muscle tissue developmentsearch RNA metabolic processsearch positive regulation of reactive oxygen species metabolic processsearch peptidyl-serine phosphorylationsearch osteoclast differentiationsearch toll-like receptor signaling pathwaysearch toll-like receptor 2 signaling pathwaysearch positive regulation of myotube differentiationsearch cellular response to lipopolysaccharidesearch cell surface receptor signaling pathwaysearch muscle cell differentiationsearch cell morphogenesissearch toll-like receptor TLR6:TLR2 signaling pathwaysearch positive regulation of transcription from RNA polymerase II promotersearch toll-like receptor 5 signaling pathwaysearch activation of MAPK activitysearch gene expressionsearch phosphorylationsearch cellular response to DNA damage stimulussearch apoptotic processsearch response to muramyl dipeptidesearch stress-activated MAPK cascadesearch cytoplasmsearch nucleoplasmsearch extracellular vesicular exosomesearch nucleussearch spindle polesearch cytosolsearch mitochondrionsearch cellsearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch