3mh0 Summary


PDB entry 3mh0 (supersedes 2pkj)

Mutagenesis of p38 MAP Kinase eshtablishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-out state

The structure was published by Bukhtiyarova, M., Karpusas, M., Northrop, K., Namboodiri, H.V., and Springman, E.B., in 2007 in a paper entitled "Mutagenesis of p38alpha MAP kinase establishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-OUT state." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 360 residues which is 100% of the natural sequence. Out of 360 residues 331 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (1-360) (MK14_HUMAN)search Homo sapienssearch 97% 360 92%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (1 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (Q16539) protein phosphorylationsearch Ras protein signal transductionsearch cell morphogenesissearch regulation of transcription, DNA-templatedsearch signal transduction in response to DNA damagesearch positive regulation of blood vessel endothelial cell migrationsearch signal transductionsearch toll-like receptor 3 signaling pathwaysearch chemotaxissearch cartilage condensationsearch regulation of sequence-specific DNA binding transcription factor activitysearch chondrocyte differentiationsearch positive regulation of transcription from RNA polymerase II promotersearch phosphorylationsearch MyD88-dependent toll-like receptor signaling pathwaysearch regulation of transcription from RNA polymerase II promotersearch cellular component movementsearch MyD88-independent toll-like receptor signaling pathwaysearch angiogenesissearch stress-induced premature senescencesearch osteoclast differentiationsearch fatty acid oxidationsearch positive regulation of erythrocyte differentiationsearch apoptotic processsearch innate immune responsesearch cellular response to vascular endothelial growth factor stimulussearch peptidyl-serine phosphorylationsearch toll-like receptor 9 signaling pathwaysearch cellular response to ionizing radiationsearch toll-like receptor TLR1:TLR2 signaling pathwaysearch striated muscle cell differentiationsearch platelet activationsearch neurotrophin TRK receptor signaling pathwaysearch positive regulation of protein import into nucleussearch TRIF-dependent toll-like receptor signaling pathwaysearch toll-like receptor 4 signaling pathwaysearch toll-like receptor 10 signaling pathwaysearch response to stresssearch positive regulation of muscle cell differentiationsearch toll-like receptor signaling pathwaysearch toll-like receptor 2 signaling pathwaysearch glucose metabolic processsearch skeletal muscle tissue developmentsearch cell surface receptor signaling pathwaysearch response to muramyl dipeptidesearch intracellular signal transductionsearch RNA metabolic processsearch transcription, DNA-templatedsearch p38MAPK cascadesearch mRNA metabolic processsearch 3'-UTR-mediated mRNA stabilizationsearch positive regulation of reactive oxygen species metabolic processsearch blood coagulationsearch MAPK cascadesearch positive regulation of myoblast fusionsearch vascular endothelial growth factor receptor signaling pathwaysearch toll-like receptor 5 signaling pathwaysearch stress-activated MAPK cascadesearch activation of MAPK activitysearch lipopolysaccharide-mediated signaling pathwaysearch response to lipopolysaccharidesearch positive regulation of myoblast differentiationsearch transmembrane receptor protein serine/threonine kinase signaling pathwaysearch positive regulation of myotube differentiationsearch cellular response to DNA damage stimulussearch gene expressionsearch DNA damage checkpointsearch negative regulation of canonical Wnt signaling pathwaysearch toll-like receptor TLR6:TLR2 signaling pathwaysearch cellular response to lipopolysaccharidesearch muscle cell differentiationsearch ATP bindingsearch transferase activity, transferring phosphorus-containing groupssearch protein bindingsearch NFAT protein bindingsearch MAP kinase activitysearch protein kinase activitysearch protein serine/threonine kinase activitysearch nucleotide bindingsearch MAP kinase kinase activitysearch kinase activitysearch transferase activitysearch nucleussearch nucleoplasmsearch cytoplasmsearch cytosolsearch extracellular vesicular exosomesearch cellsearch mitochondrionsearch spindle polesearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch