3mgy Summary


PDB entry 3mgy (supersedes 2pv5)

Mutagenesis of p38 MAP Kinase eshtablishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-out state

The structure was published by Bukhtiyarova, M., Karpusas, M., Northrop, K., Namboodiri, H.V., and Springman, E.B., in 2007 in a paper entitled "Mutagenesis of p38alpha MAP kinase establishes key roles of Phe169 in function and structural dynamics and reveals a novel DFG-OUT state." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2010.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Mitogen-activated protein kinase 14. This molecule has the UniProt identifier Q16539 (MK14_HUMAN)search. The sample contained 360 residues which is 100% of the natural sequence. Out of 360 residues 328 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Mitogen-activated protein kinase 14 Q16539 (1-360) (MK14_HUMAN)search Homo sapienssearch 97% 360 91%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q16539 (1 - 360) Mitogen-activated protein kinase 14 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (Q16539) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (Q16539) MAP kinase activitysearch ATP bindingsearch protein kinase activitysearch protein bindingsearch NFAT protein bindingsearch kinase activitysearch protein serine/threonine kinase activitysearch transferase activitysearch transferase activity, transferring phosphorus-containing groupssearch nucleotide bindingsearch MAP kinase kinase activitysearch protein phosphorylationsearch toll-like receptor TLR1:TLR2 signaling pathwaysearch regulation of transcription from RNA polymerase II promotersearch angiogenesissearch negative regulation of canonical Wnt signaling pathwaysearch osteoclast differentiationsearch chondrocyte differentiationsearch cell morphogenesissearch response to lipopolysaccharidesearch cartilage condensationsearch stress-induced premature senescencesearch toll-like receptor 5 signaling pathwaysearch innate immune responsesearch neurotrophin TRK receptor signaling pathwaysearch fatty acid oxidationsearch positive regulation of myotube differentiationsearch positive regulation of protein import into nucleussearch TRIF-dependent toll-like receptor signaling pathwaysearch chemotaxissearch transcription, DNA-templatedsearch toll-like receptor 4 signaling pathwaysearch response to muramyl dipeptidesearch intracellular signal transductionsearch MyD88-independent toll-like receptor signaling pathwaysearch platelet activationsearch positive regulation of myoblast fusionsearch positive regulation of myoblast differentiationsearch 3'-UTR-mediated mRNA stabilizationsearch toll-like receptor signaling pathwaysearch blood coagulationsearch stress-activated MAPK cascadesearch activation of MAPK activitysearch toll-like receptor 9 signaling pathwaysearch toll-like receptor 3 signaling pathwaysearch MyD88-dependent toll-like receptor signaling pathwaysearch toll-like receptor 10 signaling pathwaysearch p38MAPK cascadesearch lipopolysaccharide-mediated signaling pathwaysearch glucose metabolic processsearch toll-like receptor 2 signaling pathwaysearch regulation of transcription, DNA-templatedsearch positive regulation of blood vessel endothelial cell migrationsearch cellular response to vascular endothelial growth factor stimulussearch cellular response to lipopolysaccharidesearch signal transductionsearch muscle cell differentiationsearch positive regulation of muscle cell differentiationsearch response to stresssearch positive regulation of reactive oxygen species metabolic processsearch peptidyl-serine phosphorylationsearch skeletal muscle tissue developmentsearch phosphorylationsearch positive regulation of transcription from RNA polymerase II promotersearch cellular component movementsearch positive regulation of erythrocyte differentiationsearch cellular response to DNA damage stimulussearch toll-like receptor TLR6:TLR2 signaling pathwaysearch DNA damage checkpointsearch myoblast differentiation involved in skeletal muscle regenerationsearch cellular response to ionizing radiationsearch Ras protein signal transductionsearch RNA metabolic processsearch apoptotic processsearch cell surface receptor signaling pathwaysearch mRNA metabolic processsearch signal transduction in response to DNA damagesearch striated muscle cell differentiationsearch transmembrane receptor protein serine/threonine kinase signaling pathwaysearch gene expressionsearch vascular endothelial growth factor receptor signaling pathwaysearch regulation of sequence-specific DNA binding transcription factor activitysearch nucleoplasmsearch extracellular vesicular exosomesearch nucleussearch cytosolsearch cytoplasmsearch mitochondrionsearch spindle polesearch cellsearch

Chain InterPro annotation
A Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Mitogen-activated protein (MAP) kinase, conserved sitesearch Mitogen-activated protein (MAP) kinase, p38search Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch