› 3mf3

X-ray diffraction
2.5Å resolution

Cobalt(II)-Substituted Haemophilus influenzae B-Carbonic Anhydrase

Released:
DOI: 10.2210/pdb3mf3/pdb
PDB entry 3mf3 coloured by chain, front view.
PDB entry 3mf3 coloured by chain, side view.
PDB entry 3mf3 coloured by chain, top view.
Source organism: Haemophilus influenzae
Entry authors: Hoffmann KM, Rowlett RS

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 221 amino acids
Theoretical weight: 25.32 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-221; Coverage: 97%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand CO 6 x CO
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 229.492Å b: 144.929Å c: 52.215Å
α: 90° β: 93.77° γ: 90°
R-values:
R R work R free
0.176 0.173 0.228
Expression system: Escherichia coli

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