› 3md0

X-ray diffraction
2.45Å resolution

Crystal structure of arginine/ornithine transport system ATPase from Mycobacterium tuberculosis bound to GDP (a RAS-like GTPase superfamily protein)

Released:
DOI: 10.2210/pdb3md0/pdb
PDB entry 3md0 coloured by chain, front view.
PDB entry 3md0 coloured by chain, side view.
PDB entry 3md0 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Edwards TE, Baugh L, Bullen J, Baydo RO, Witte P, Thompkins K, Phan IQ, Abendroth J, Clifton MC, Sankaran B, Van Voorhis WC, Myler PJ, Staker BL, Grundner C, Lorimer DD
J Struct Funct Genomics 16 91-9 (2015)
PMID: 25832174

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162031 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable GTPase Rv1496 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 38.6 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPZ1 (Residues: 3-336; Coverage: 99%)
Gene names: MTCY277.18, Rv1496
Sequence domains: Methylmalonyl Co-A mutase-associated GTPase MeaB
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 65.53Å b: 187.36Å c: 66.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.251
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
1 more

4 mentions without citation

Mycobacterium thermoresistibile as a source of thermostable orthologs of Mycobacterium tuberculosis proteins.
Edwards et al. (2012)
3 more